相关实验视频

Updated: Jun 28, 2025

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

通过整合scRNA-seq,空间转录组和大量RNA-seq数据来准结直肠癌中的核酸代谢途径

Songyun Zhao^1,2, Pengpeng Zhang³, Sen Niu^1,4

¹Wuxi Medical Center of Nanjing Medical University, Wuxi, China.

Functional & integrative genomics

|April 9, 2024

概括

这项研究表明,结直肠癌 (CRC) 细胞中核酸代谢的改变与疾病进展和患者的结果有关. 鉴定关键的核酸代谢相关基因 (NMRGs) 为CRC提供了新的预后标记物和治疗点的潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

大肠直肠癌 (CRC) 是一种主要的消化系统恶性瘤.
核酸代谢对于CRC进展和治疗策略至关重要.

研究的目的:

研究核酸代谢在CRC异质性中的作用.
确定新型预后标记物和CRC的治疗点.

主要方法:

单细胞RNA测序 (scRNA-seq) 和空间转录组测序 (stRNA-seq) 用于分析核酸代谢.
使用TCGA和GEO数据开发了一个预后风险模型.
在体外实验验证了关键基因和治疗点.

主要成果:

CRC表现出细胞异质性,在髓状和上皮细胞中核酸代谢升高.
观察到高新陈代谢的上皮细胞和纤维细胞之间的通信增强.
一个四基因预后模型准确地预测了患者的生存时间和对治疗的反应.
ACOX1和CPT2被确定为CRC的潜在治疗点.

结论:

与核酸代谢相关的基因 (NMRGs) 在CRC中具有重要意义.
开发的预后模型显示了个性化CRC治疗的前景.
ACOX1和CPT2代表着结直肠癌的新型治疗点.

关键词:

结肠直肠癌是一种癌症.免疫治疗是一种免疫疗法.核酸代谢的核酸代谢预测模型是一个预测模型.这就是scRNA-seqq.这就是stRNA-seqq.

更多相关视频

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...