Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Development of Blood Vessels01:07

Development of Blood Vessels

The development of the vascular system in a fetus is a complex and intricate process that begins as early as 15 to 16 days post-conception. This process starts outside the embryo, specifically in the mesoderm of the yolk sac, chorion, and connecting stalk. Approximately two days later, the formation of blood vessels occurs within the embryo itself.
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Loss of STMP1 impairs mitochondrial structure and links inflammaging to heart failure.

Nature cardiovascular research·2026
Same author

Metabolic Profiling Identifies Infertile Men at Risk of Accelerated Aging: Implications for Systemic and Reproductive Health.

Andrology·2026
Same author

A world of possibilities for cardiac simulations.

Nature cardiovascular research·2026
Same author

Selection of human hematopoietic stem cells bearing the intended functional edit by transient AND-gate reporters.

Nature biotechnology·2026
Same author

Ex vivo expansion of hematopoietic stem and progenitor cells from human mobilized peripheral blood for gene therapy applications.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Publisher Correction: The NPR1 agonist XXB750 may worsen heart failure.

Nature cardiovascular research·2026

相关实验视频

Updated: Jun 19, 2026

Isolation of Murine Embryonic Hemogenic Endothelial Cells
08:56

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Published on: June 17, 2016

11.5K

在人类胚胎发育过程中,CD32捕获受影响的血源性内皮细胞.

Rebecca Scarfò1, Lauren N Randolph1, Monah Abou Alezz1

  • 1San Raffaele Telethon Institute for Gene Therapy, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Nature cell biology
|April 9, 2024
PubMed
概括

研究人员确定CD32 (FCGR2B) 是人类胚胎中血源性内皮细胞 (HEC) 的标记物. 这一发现使得能够精确地隔离HECs,以便在体外有效地生成造血细胞.

更多相关视频

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

7.2K
Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells
04:23

Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

Published on: March 31, 2021

2.2K

相关实验视频

Last Updated: Jun 19, 2026

Isolation of Murine Embryonic Hemogenic Endothelial Cells
08:56

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Published on: June 17, 2016

11.5K
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
11:00

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

7.2K
Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells
04:23

Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

Published on: March 31, 2021

2.2K

科学领域:

  • 发展生物学 发展生物学
  • 血液形成 血液形成 血液形成
  • 细胞生物学 细胞生物学

背景情况:

  • 血细胞源自专门的内皮细胞,称为血源内皮细胞 (HECs).
  • 在早期人类胚胎中,HECs是罕见的和短暂的,因此很难将它们与其他内皮细胞区分开来.

研究的目的:

  • 确定一种可靠的标记物,用于从人类胚胎中分离出血源性内皮细胞 (HEC).
  • 了解HECs的发育轨迹及其对血液形成的承诺.

主要方法:

  • 对人类胚胎内皮细胞 (28至32天胚胎) 的转录基因分析.
  • 使用人类胚胎干细胞衍生的内皮细胞进行功能性检测.
  • 流细胞计用于识别内皮细胞上的CD32表达.

主要成果:

  • 在含有HECs的内皮细胞中,FC受体CD32 (FCGR2B) 的表达高度丰富.
  • CD32阳性 (CD32+) 内皮细胞表现出强大的多系细胞形成潜力.
  • 90%的CD32+内皮细胞被确定为真诚的HEC.
  • FCGR2B表达标志着HECs对血液生成命运的不可逆转的承诺点.

结论:

  • CD32是一种精确的标记物,用于从人类胚胎和多能干细胞衍生的内皮细胞中分离HECs.
  • 这一发现促进了血液细胞的高效体外生成.