相关实验视频

Updated: Jun 28, 2025

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

阐明EPPK1在肺腺癌发展中的作用

Ken Arimura^1,2, Michael Kammer¹, S M Jamshedur Rahman¹

¹Division of Allergy, Pulmonary and Critical Care Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.

|April 9, 2024

概括

埃皮普拉金1 (EPPK1) 在肺腺癌 (LUAD) 进展中起作用,与吸烟有关. 淘汰EPPK1会抑制癌症的生长和入侵,这表明它是癌症.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症遗传学癌症遗传学

背景情况:

在12%的肺腺癌 (LUAD) 病例中发现了Epiplakin 1 (EPPK1) 变化.
EPPK1的改变与早期LUAD的不良预后相关.
在癌症发展中EPPK1的确切作用需要进一步研究.

研究的目的:

调查白蛋白1 (EPPK1) 在肺腺癌 (LUAD) 发展中的作用.
为了确定在LUAD细胞系中EPPK1淘汰后的功能和遗传变化.

主要方法:

与临床变量相关的EPPK1mRNA和蛋白质表达的分析.
将正常的支气管上皮细胞暴露在香烟烟雾中,以评估EPPK1表达变化.
在LUAD细胞系中通过CRISPR-Cas9调解EPPK1的淘汰,以评估功能和遗传影响.

主要成果:

EPPK1蛋白表达与吸烟史和早期LUAD的不良预后有关.
EPPK1淘汰 (KO) 诱导了介质细胞到上皮细胞的过渡,减少了细胞的增殖和侵入.
EPPK1 KO改变了参与瘤发生,亡,血管生成和细胞生长的关键基因的表达,包括MYC和p53.

结论:

EPPK1涉及与吸烟相关的LUAD,上皮细胞转变为介质细胞,以及癌症进展.
EPPK1在调节癌症进展中的作用表明它有可能成为LUAD的治疗点.
对EPPK1的机制的进一步研究可能会导致新的LUAD治疗策略.

关键词:

克里斯普尔-Cas9是什么意思爱皮拉基因 1 1 从上皮细胞转变为介质细胞的过渡.在MYC/p53路径中.

更多相关视频

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Published on: March 3, 2023

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

相关概念视频

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Tumor Progression

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...