长非编码RNAUNC5B-AS1通过在多形质母细胞瘤中海绵化miR-24-3p抑制细胞增殖
Ying Song1, Baodong Chen2, Huili Jiao1
1Department of Neurology, Peking University Shenzhen Hospital, Shenzhen, 518036, China.
BMC medical genomics
|April 9, 2024
概括
我们发现了一种新的长非编码RNA (lncRNA) 签名,UNC5B-AS1,可以抑制多种质母细胞瘤 (GBM) 细胞生长. 这种lncRNA与miR-24-3p相互作用,为GBM提供潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 多形质母细胞瘤 (GBM) 是一种高度攻击性的中枢神经系统原发性瘤,结果不佳.
- 关于长非编码RNA (lncRNA) 签名及其微RNA (miRNA) 在GBM中的相互作用的现有知识仍然有限.
- 识别新型lncRNA标记物和了解它们的生物功能对于GBM研究至关重要.
研究的目的:
- 在多种质母细胞瘤中发现新的lncRNA特征及其相关的miRNA.
- 阐明已识别的lncRNA及其监管网络在GBM中的功能作用.
- 探索这些发现对GBM诊断和治疗开发的潜力.
主要方法:
- 利用来自癌症基因组图谱 (TCGA) 的RNA测序数据进行GBM分析.
- 使用"边缘"R包确定差异表达的lncRNAs (DELs).
- 预测了lncRNA-miRNA与Cytoscape的相互作用,并通过starBase和GSEA分析了miRNA存活率和基因丰富 (KEGG).
- 使用细胞计数工具-8 (CCK-8) 测定UNC5B-AS1.1.的细胞增殖.
主要成果:
- 发现unc-5网林受体B反感RNA1 (UNC5B-AS1) 的过度表达抑制了GBM细胞的增殖.
- UNC5B-AS1的抗增殖作用与其与miR-24-3p.p.的相互作用有机学的联系.
- 在GBM中确定了一个涉及UNC5B-AS1和miR-24-3p的新型监管网络.
结论:
- 发现UNC5B-AS1-miR-24-3p网络为lncRNAs和miRNAs在GBM病原发生中的作用提供了新的见解.
- 这一网络对改善GBM患者临床预后评估具有潜在意义.
- 已确定的lncRNA-miRNA轴为开发针对GBM的新型治疗策略提供了一个有前途的途径.
相关概念视频
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
The Retinoblastoma Gene
4.1K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
Negative Regulator Molecules
35.4K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.4K


