在加纳的污水和废水中首次分子检测到SARS-CoV-2
Ewurabena Oduma Duker1, Evangeline Obodai1, Seth Offei Addo2
1Virology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
BioMed research international
|April 10, 2024
概括
在加纳17%的废水和污水样本中检测到严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) RNA. 虽然病毒RNA持续存在,但传染性SARS-CoV-2并未被隔离,这表明环境样本中的非传染性.
科学领域:
- 环境科学环境科学
- 病毒学 病毒学
- 公共卫生 公共卫生
背景情况:
- 严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 在便中流出.
- 污水监测是监测社区病毒存在的工具.
研究的目的:
- 在加纳的污水和废水中检测SARS-CoV-2.
- 在这些环境矩阵中评估SARS-CoV-2的潜在生存和传染性.
主要方法:
- 涉及加纳7个地区16个地点的354个废水和污水样本的横截面研究 (2020年11月至2022年11月).
- 为检测SARS-CoV-2进行实时PCR.
- 下一代测序 (NGS) 用于变种表征.
- 试图在Vero细胞系中隔离病毒.
主要成果:
- 17%的样本检测出SARS-CoV-2RNA的阳性 (6%的污水,11%的废水).
- 在阿克拉大区 (7.3%) 患病率最高.
- 发现了阿尔法 (B.1.1.7) 和德尔塔 (AY.36) 变种.
- 由于病毒载量低 (Ct>35) 或高温,SARS-CoV-2的隔离没有成功,这可能是由于病毒载量低 (Ct>35) 或高温.
结论:
- 在加纳的废水和污水中,SARS-CoV-2 RNA 很普遍.
- 这些样本中的环境SARS-CoV-2RNA可能不具有传染性.
- 调查结果表明,在加纳社区内,SARS-CoV-2的传播持续.
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