微毛细管网络的差异性蛋白质组学特征,以响应声音模式驱动的局部细胞密度增强
N Di Marzio1,2, R Tognato1, E Della Bella1
1AO Research Institute Davos, 7270 Davos, Switzerland.
Biomaterials and biosystems
|April 10, 2024
概括
声音模式增强了局部细胞密度,促进了微毛囊网络在体外的形成. 这种生物制造技术改变了蛋白质表达,为组织工程和先进的体外模型提供了洞察力.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 空间细胞组织在体外生物制造微毛细管网络中至关重要.
- 目前在生物制造中控制细胞密度的方法有限.
研究的目的:
- 研究基于声音的局部细胞密度增强对微毛细管网络形成的影响.
- 分析受声音诱导凝结影响的细胞的蛋白质组概况.
主要方法:
- 人类静脉内皮细胞 (HUVEC) 和人体皮质细胞 (hPC-PL) 在纤维素中悬浮.
- 声音诱导的水力动力学力被用于细胞模式和凝结.
- 进行了蛋白质组分析,基因本体学 (GO) 丰富和发明路径分析 (IPA).
主要成果:
- 声音模式有效地将细胞凝结成所需的几何形状,产生显著的局部细胞密度梯度.
- 沿着这些梯度形成了毛细管状结构,维持了五天的模式.
- 确定了900多种差异表达的蛋白质,其中VEGF-A被认为是关键的上游调节器.
结论:
- 声音模式是一种可行的生物制造技术,用于创建有组织的微毛细管网络.
- 细胞通过声音的凝结会影响分子和蛋白质的形状,影响网络的形成.
- 这些发现提升了对复杂的体外模型和组织工程的声音模式的理解.
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