经过实验验证的批量和单细胞转录组将USP18确定为一种新的质瘤预后和扩散指标
1Department of Neurosurgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
Experimental and therapeutic medicine
|April 10, 2024
概括
在质瘤中抑制泛素特异性蛋白酶18 (USP18) 会阻碍癌细胞的增殖. USP18 Knockdown可以通过干扰素I型 (IFN-I) 响应调节来降低质瘤细胞的活力和克隆原性.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 在癌症中,乌比基因特异性蛋白酶18 (USP18) 抑制通过干扰素 (IFN) 刺激的基因促进了热.
- USP18影响质瘤细胞上皮层-介质细胞过渡,并与预后不佳有关.
- USP18水平对质瘤患者的免疫疗法反应产生影响.
研究的目的:
- 研究USP18在质瘤进展中的作用及其与患者预后和免疫治疗反应的关联.
- 确定USP18敲击对质瘤细胞活力和增殖的影响.
- 阐明USP18影响结质瘤细胞的机制,特别是关于IFN-I反应.
主要方法:
- 对质瘤样本的批量和单细胞转录组分析.
- 对USP18表达与患者预后和免疫治疗数据的相关性分析.
- 在U251和U87MG质瘤细胞系中进行USP18敲击.
- 细胞计数工具-8试验和克隆原性试验,以评估细胞活力和增殖.
主要成果:
- USP18在质瘤上升调节,并与预后不佳相关.
- USP18表达水平与质瘤T细胞的免疫疗法反应和1型IFN反应相关.
- USP18 Knockdown显著降低了质瘤细胞的活力,并抑制了克隆性.
结论:
- 在USP18中, Knockdown抑制了质瘤细胞的增殖,这表明它是潜在的治疗点.
- USP18对质瘤扩散的影响可能通过其对IFN-I反应的影响来调解.
- 针对USP18可能是一个可行的策略,以改善质瘤治疗结果.
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Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
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