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Updated: Jun 28, 2025

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从解决方案到结构:通过生物样本的预表征管道赋予包容性冷EM权力

Christoph Mueller-Dieckmann1, Alessandro Grinzato1, Grégory Effantin2

  • 1Structural Biology, European Synchrotron Radiation Facility (ESRF), 71 Avenue des Martyrs, Grenoble 38000, France.

Journal of applied crystallography
|April 10, 2024
PubMed
概括

低温电子显微镜 (cryo-EM) 解决方案到结构 (SOS) 管道为没有先进的低温电子显微镜基础设施的研究人员提供了全面的服务. 该管道通过提供从样本准备到高分辨率数据收集的端到端支持,使结构生物学研究民主化.

关键词:
在SOS管道SOS管道.低温电磁波冷却器 (Cryo-EM) 是一个非常好的方法.冷电子显微镜的使用方法冷电子断层扫描 (Cryo-electron tomography) 是一种使用冷电子断层扫描的技术.一个粒子分析分析.玻璃化 玻璃化 玻璃化

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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