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一个优化的基因型化工作流程,用于识别高度SCRaMbLEd合成酵母

Timon A Lindeboom¹, María Del Carmen Sanchez Olmos¹, Karina Schulz²

¹Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043 Marburg, Germany.

ACS synthetic biology

|April 10, 2024

概括

合成酵母菌株 (Sc2.0) 可以使用SCRaMbLE技术产生基因组多样性. 新的qPCR原型 (loxTags) 和原型预测工具 (qTagGer) 能够有效地对这些多样化的酵母种群进行基因定型.

科学领域:

合成生物学合成生物学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

合成酵母菌株 (Sc2.0) 拥有众多的重组位点 (loxPsym),可以通过SCRaMbLE进行基因组重组.
SCRaMbLE技术可以从单个基因型中产生高度多样化的酵母种群.

研究的目的:

开发一种有效的工作流程,用于选择具有重新排列合成染色体的基因多样化的酵母候选人.
介绍 loxTags,用于基因型定位 loxPsym 站点的 qPCR 启动器,以及启动器预测工具 qTagGer.

主要方法:

开发了 loxTags,一组用于跨 loxPsym 站点的基因类型定型的 qPCR 启动器.
创建了qTagGer,这是一个qPCR基因型化原始预测工具,用于管理众多的amplicons.
使用基于loxTag的基因型和长读序列.

主要成果:

loxTags可以在SCRaMbLE之后检测删除,反转和转位.
光诱导克雷复合酶 (L-SCRaMbLE) 在Sc2.0菌株中有效地产生了各种复合事件.
在合成染色体III (线性和圆形版本) 上成功应用.

结论:

loxTags和qTagGer为分析合成酵母中SCRaMbLE诱导的基因组多样性提供了高效的工作流程.
在Sc2.0酵母菌株中产生复杂的基因组重组时,L-SCRaMbLE是有效的.

关键词:

标签:PCRTags 标签:PCRTags 标签:PCRTags 这是一个可靠的SCRAMBLE.基因组重新排列基因组重新排列视觉遗传学视觉遗传学 qPCR的基因型鉴定它们被重新组合起来.合成生物学合成生物学合成酵母是一种合成酵母.

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