桥梁离子电流校正和电阻脉冲传感可用于可靠的宽线性检测
Xian Zhang1, Zeng-Qiang Wu2, You-Wei Zheng1
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Analytical chemistry
|April 10, 2024
概括
这项研究引入了一种新的纳米孔传感方法,将离子电流校正 (ICR) 和电阻脉冲传感 (RPS) 结合起来,用于可靠的miRNA检测. 该技术实现了从1 fM到1 nM的广泛线性检测范围,即使在复杂的生物样本中也是如此.
科学领域:
- 纳米孔感应 纳米孔感应
- 生物分子检测检测
- 分析化学是一种分析化学.
背景情况:
- 离子电流纠正 (ICR) 由于信号波动,在痕迹检测方面存在局限性.
- 电阻脉冲传感 (RPS) 在高度样本中面临着孔隙堵塞的挑战.
- 需要一种统一的纳米孔探测方法来克服单个技术的局限性.
研究的目的:
- 开发一个双模纳米孔探测战略,整合ICR和RPS.
- 为了实现微RNA (miRNA) 的可靠和灵敏的检测,具有广泛的线性范围.
- 克服ICR中微量水平波动和RPS中高度堵塞的局限性.
主要方法:
- 纳米孔大小与DNA四面体 (TDN) 结构的合理匹配.
- 利用miRNA-10b的TDN的特定结合和释放来调节纳米孔信号.
- 同时测量由分析物-TDN相互作用引起的不同的ICR和RPS信号.
主要成果:
- 实现了miRNA-10b从1 fM到1 nM的广泛线性检测范围.
- 证明了与孔状几何形状和表面电荷调制相关的独特ICR信号.
- 通过纳米孔通过miRNA-10b-TDN复合体产生的观察到的RPS信号.
- 在单细胞和真实血样本中验证了该方法的可行性.
结论:
- 集成ICR和RPS纳米孔传感方法提供了增强的可靠性和线性.
- 这种方法有效地解决了传统ICR和RPS技术的局限性.
- 开发的生物传感器在复杂的生物环境中显示出敏感miRNA检测的前景.
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