尿酸的色度测量和度测定通过基于悬浮的测定,使用酶固定微型颗粒
Yukiko Moriiwa1, Keigo Hatakeyama1, Kazuhiro Morioka1
1Department of Biomedical Analysis, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan.
概括
一种新的基于悬浮的测定方法可以精确地每天监测血清尿酸水平. 这种方法使用酶固定微粒进行增强的色度分析,有助于高尿血症管理.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 每天对血清尿酸的监测对于管理高尿血症至关重要.
- 目前的方法可能缺乏频繁的个人监测的敏感性或实用性.
研究的目的:
- 开发一种新的,灵敏的,视觉上优异的测试方法来测量血清尿酸.
- 为了使尿酸的定量分析使用随时可用的微板阅读器.
主要方法:
- 一种基于悬浮的测定方法,使用微粒与尿和胡卜过氧化酶 (HRP) 固定.
- 基于从AMPLEX红色中产生Resorufin的色度检测,它被吸附在微粒上.
- 通过乙醇诱导的溶解和光检测实现的定量测量.
主要成果:
- 该测试表明,由于微粒上缩的resorufin,可见度更高.
- 使用标准的96孔光微板阅读器,量化分析是可行的.
- 达到了2.2 × 10-2μg/mL的检测极限,相对标准偏差 (RSD) ≤12.1%.
结论:
- 开发的测定方法为日常血清尿酸监测提供了一种敏感和实用的方法.
- 这种方法为高尿素血症患者提供了个性化治疗策略.
- 该方法对便携式度仪的适应性表明,该方法有可能用于护理点诊断.
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