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[精子等离子体外体和精子DNA碎片化指数:一种代谢学分析]

Bao-Hao Liu^1,2, Yong Sun², Qing-Hua Zhang²

¹Suzhou Medical College of Soochow University, Suzhou, Jiangsu 215123, China.

Zhonghua nan ke xue = National journal of andrology

|April 10, 2024

概括

精子DNA碎片化 (DFI) 高的男性具有显著较低的精液等离子体乙卡尼丁水平. 这一发现表明,乙卡尼丁可以作为一个生物标志物来定量和快速评估精子DNA损伤.

科学领域:

生殖生物学生殖生物学
代谢学代谢学代谢学
精子健康精子健康

背景情况:

精子DNA碎片化 (DFI) 是男性不孕症的一个关键因素.
精子等离子体外体 (SPE) 含有反映精子健康的生物标志物.
了解与DFI相关的SPE的代谢差异对于诊断至关重要.

研究的目的:

为了比较高和低精子DNA碎片化指数 (DFI) 的男性之间的精子等离子体外体 (SPE) 中的代谢物水平.
为了确定评估精子DNA损伤的潜在生物标志物.

主要方法:

对两组男性进行了精子外体代谢分析:低DFI (≤15%) 和高DFI (≥30%).
使用高性能液态染色学和质谱学来识别和量化代谢物.
基因和基因组的京都百科全书 (KEGG) 途径分析进行.

主要成果:

确定了与精子DNA完整性相关的23种代谢物,包括有机酸,氨基酸中间体和甲.
基因基因基因基因基因分析表明,它涉及到螺旋体信号传递,尼亚和阿尔金因/氨酸代谢.
与对照人群相比,在高DFI的男性中观察到精液等离子酸的水平明显较低.

结论:

在SPE中,有机酸,氨基酸和甲酸在高和低DFI的男性之间存在显著的差异.
精液血乙卡尼丁水平与精子DFI有显著的相关性.
乙卡尼丁可以作为评估精子DNA损伤的定量和快速指标.

关键词:

精子等离子体外体; DNA 损伤; 精子 DNA 碎片化指数; 代谢学; 乙卡尼丁.

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