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主机-客人识别-介导的超分子聚合-诱导的电化学发光的伊 (III) 复合物用于核酸生物测试

Jinwen Zhao¹, Xingrong Tan², Ying He¹

¹Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.

Analytical chemistry

|April 10, 2024

概括

这项研究引入了一种新的聚合诱导电光发射 (AIECL) 的超分子系统,使用宿主-客人识别,提高发射器的稳定性和效率. 该系统通过DNA步行机制实现了对miRNA-16的超敏感检测.

科学领域:

材料科学材料科学材料科学
超分子化学超分子化学
分析化学分析化学
生物技术是生物技术.

背景情况:

现有的聚合诱导电发光 (AIECL) 发射器通常由于静电集成而遭受低效率和结构稳定性差.
需要先进的AIECL材料,提高性能和稳定性,用于实际应用.
电化学发光 (ECL) 传感为生物分子检测提供了高灵敏度,但需要强大的发射系统.

研究的目的:

开发一种新的超分子AIECL发射器,使用宿主-客人识别来克服传统静电集成方法的局限性.
为微RNA-16 (miRNA-16) 检测构建一个高度敏感和稳定的ECL传感平台.
探索超分子AIECL在先进生物试验开发中的潜力.

主要方法:

通过宿主-客人识别,通过库库比特[8]uril (CB[8]) 和tris(2-phenylpyridine) (III) ([Ir(ppy) ₃) 之间制造一个超分子复合体 (Ir-CB[8]).
通过将Ir-CB[8]发射器与miRNA-16.6触发的DNA步行机制相合,开发了一个ECL传感系统.
使用Au纳米颗粒@norepinephrine (AuNPs@NE) 作为灭剂,这些纳米颗粒被DNA步行器所取代,以恢复ECL信号以进行检测.

主要成果:

与静电方法相比,超分子复合体Ir-CB[8]表现出增强的AIECL性能,具有更好的结构稳定性和效率.
开发的ECL传感系统证明了miRNA-16的超敏感检测,利用类似"风车"的DNA步行者的释放火器的能力.
该系统显示出高效率和生物相容性,在目标miRNA-16存在时恢复ECL排放.

结论:

主机-客人识别介导的超分子AIECL为创建稳定和高效的AIECL发射器提供了有前途的战略.
结合Ir-CB[8]的DNA步行系统代表了基于ECL的生物传感在miRNA检测中的重大进步.
这种方法为开发各种生物试验的先进ECL传感平台提供了新的途径.

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