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目标部位和非目标部位的抵抗机制赋予了Alopecurus aequalis中的中硫甲基耐药性

You Zhan¹, Haozhe Liu¹, Ziheng Cao¹

¹College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China.

Plant physiology and biochemistry : PPB

|April 10, 2024

概括

短城狐尾因位突变和新陈代谢增加而对甲基硫除草剂产生了高耐药性. 这种耐药性涉及单氧化酶和谷氨S转移酶.

科学领域:

农业科学农业科学
植物科学植物科学
生物化学生物化学

背景情况:

短城狐尾 (Alopecurus aequalis) 是中国农业的一个重要杂草.
广泛使用抑制乙酸盐合成酶 (ALS) 的除草剂导致了A. aequalis的快速耐药性发展.
在中国的一片小麦田中发现了一种对甲 (Aa-R) 耐药的种群.

研究的目的:

为了研究Shortawn狐尾 (A. aequalis) 的甲基硫耐药群体中的耐药机制.
确定导致这种杂草物种对除草剂耐药性的遗传和生化因素.

主要方法:

进行了剂量反应测试,以评估对各种ALS抑制除草剂的耐药性水平.
对突变的乙烯酸乳酸合成酶 (ALS) 基因序列的分析.
对ALS,细胞染色体P450单氧化酶 (CYP450),和谷氨S转移酶 (GST) 基因的基因表达分析.
酶活性测定和甲基硫甲基的代谢研究.
分子对接模拟. 分子对接模拟.

主要成果:

亚-R群体对甲基硫具有高耐药性,对其他ALS抑制剂具有中度敏感性.
在ALS基因中发现了一种已知的耐药性突变 (Pro-197-Thr) 及其过度表达.
耐药性被CYP450和GST抑制剂部分逆转,表明非位耐药性.
在 Aa-R 群体中观察到甲硫甲基的增强代谢和可诱导的 CYP450 和 GST 活动.
证实了CYP74A2和GST4基因的上调以及它们与除草剂的结合亲和力.

结论:

通过ALS基因的突变和过度表达而产生的点抗性是主要的机制.
通过CYP450和GST酶促进新陈代谢的非目标部位耐药性也起着至关重要的作用.
目标部位和非目标部位的联合耐药机制有助于A. aequalis中高水平的甲基硫耐药性.

关键词:

这种植物是Alopecurus aequalis.细胞染色体P450单氧化原酶.谷氨酸S转移酶是什么中硫甲基-甲基硫.非目标站点电阻的阻力.

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