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在MS患者中,ocrelizumab B细胞枯竭对PBMC中的HERV RNA表达没有影响

Rachael Tarlinton¹, Radu Tanasescu², Claire Shannon-Lowe³

¹School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, United Kingdom.

Multiple sclerosis and related disorders

|April 10, 2024

概括

多发性硬化症 (MS) 的ocrelizumab治疗没有改变爱斯坦-巴尔病毒 (EBV) 或人类内源逆转录病毒 (HERV) 的表达. 虽然与MS发作有关,但这些病毒载量不受ocrelizumab治疗的显著影响.

科学领域:

神经免疫学神经免疫学
病毒学病毒学
基因组学就是基因组学.

背景情况:

爱斯坦-巴尔病毒 (EBV) 感染是多发性硬化症 (MS) 的关键触发因素.
人体内源逆转录病毒 (HERVs),特别是HERV-W,被认为是MS风险因素.
奥克雷利祖马布是一种B细胞消耗疗法,针对MS治疗的CD20.

研究的目的:

研究ocrelizumab对MS患者基因表达的影响.
为了评估爱斯坦-巴尔病毒 (EBV) 和人类内源性逆转录病毒 (HERV) 负载的变化后的ocrelizumab治疗.
为了测试EBV和HERV-W负载在ocrelizumab治疗后降低的假设.

主要方法:

从治疗前和治疗后的多发性硬化症患者样本对联的外周血液单核细胞 (PBMC) 的RNA测序.
使用鱼和DeSeq2.2进行差异基因表达分析.
针对EBV和HERV数据库进行特定映射,以量化病毒RNA.

主要成果:

患者样本按个人分组,而不是按治疗或疾病类型.
14个与B细胞相关的基因在治疗后显著下调.
在治疗前和治疗后的样本之间没有观察到EBV或HERV表达的显著变化.

结论:

EBV和HERV的表达与MS病原体的发起有关,而不是其正在进行的过程.
在MS患者中,ocrelizumab治疗似乎不会改变EBV或HERV表达水平.
高水平的病毒表达不是由ocrelizumab影响的活跃MS疾病状态的特征.

关键词:

爱斯坦巴尔病毒病毒.人体内源性逆转录病毒多发性硬化症是多发性硬化症.这就是Ocrelizumab.

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