在使用STR和SNP位点组合进行亲属测试时,确定遗传联系的影响
Da Yang1, Sheng Xuan Ma2, Guo Liang Zhao2
1Institute of Forensic Medicine And Laboratory Medicine, Jining Medical University, Shandong province, P. R. China; Forensic Science Center of Jining Medical University, Shandong province, P. R. China.
Legal medicine (Tokyo, Japan)
|April 10, 2024
概括
遗传联系对法医亲属关系测试中的整体概率比率 (LR) 分布有很小的影响,但可以显著影响例行病例. 考虑链接可以提高遗传标记的歧视力.
科学领域:
- 法医遗传学 法医遗传学
- 人口遗传学 人口遗传学
- 生物技术是生物技术.
背景情况:
- 血统概率 (LR) 对于法医亲属测试至关重要,利用来自短并列重复 (STR) 和单核酸多态 (SNP) 位点的DNA数据.
- 生物技术的进步增加了遗传标记物的数量,扩大了亲属关系分析的范围.
- 靠近位置的基因位点之间的遗传联系是遗传标记分析的固有因素.
研究的目的:
- 研究遗传联系对不同程度的亲属关系 (第一,第二和第三度) 的亲属关系测试的影响.
- 以 (LRlinkage) 和没有 (LRignore) 考虑遗传联系计算的概率比较.
主要方法:
- 利用使用Lander-Green算法的GeneVisa软件来模拟亲属测试场景.
- 模拟了LRlinkage和LRignore值,使用来自欧洲人口的43个STR和134个SNP的数据.
- 分析了等位基因频率和遗传距离数据,以评估对LR分布和例行病例LR的联系效应.
主要成果:
- 遗传联系对概率比率的总体分布的影响被发现是最小的.
- 然而,遗传联系在常规亲属测试案例中对概率比率产生了重大影响.
- 考虑到遗传联系被证明可以增强在亲属关系确定中使用的遗传标记者的歧视力.
结论:
- 虽然对LR分布的链接效应很小,但在实际的法医亲属测试中,它们可能很重要.
- 结合遗传联系分析可以提高亲属测试的准确性和功率.
- 基因Visa软件为分析法医亲属关系调查中的遗传联系提供了一个强大的平台.
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