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多功能光探针用于同时揭示ferroptosis中的Cys和ONOO动态相关性

Xiongbo Liu¹, Jiali Zhu¹, Qiangsheng Zhang²

¹Hainan Provincial Key Laboratory of Fine Chem, School of Chemistry and Chemical Engineering, Hainan University, Haikou 570228, China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|April 10, 2024

概括

研究人员开发了一种新的光探针,可以在铁灭过程中同时检测氨酸和过氧酸盐. 探测器显示,当诱导铁亡时,囊蛋白的减少和过氧酸盐的增加,为氧化还原调节提供了新的见解.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
化学生物学化学生物学

背景情况:

铁亡是一种受调节的细胞死亡途径,由脂质过氧化驱动,与铁代谢和氧化还原失衡有关.
了解铁灭菌需要研究反应性氧物种 (ROS) 和反应性硫物种 (RSS) 之间的相互作用.
半氨酸 (Cys) 和过氧酸盐 (ONOO-) 是关键的氧化还原活性分子,参与细胞过程,包括铁灭.

研究的目的:

开发一种新的光探针,用于同时检测细胞内氨酸和过氧酸盐.
为了研究在埃拉斯诱导的铁亡过程中氨酸和过氧酸盐之间的动态相互作用.
阐明氧化还原调节在铁灭调节中的作用.

主要方法:

一种库马林染料的结合,具有氨酸和过氧酸盐的特定反应部位.
开发一种双通道光探针 (LXB),用于同时检测Cys和ONOO- (Cys发射在498nm,ONOO-发射在565nm).
监测细胞内Cys和ONOO-水平,以诱导铁亡的目的用埃拉斯治疗的细胞中.

主要成果:

LXB探测器成功地实现了Cys和ONOO-的同时检测,它们具有明显的光通道.
埃拉斯诱导的铁亡导致了快速生成ONOO-和细胞内Cys水平的显著下降.
观察到囊水平最初下降,然后在铁亡进展期间稳定在低水平.

结论:

这项研究介绍了第一个光探针,用于实时,同时监测铁死中的氨酸和过氧酸盐.
这些发现突显了铁灭过程中囊缺乏和过氧酸盐生成之间的动态相互作用.
结果提供了关于氧化还原控制机制的宝贵见解,以及Cys和ONOO-在铁灭中的潜在相互相关性.

关键词:

Cys 和 ONOO () 的意思.它是双通道的.铁性化 (ferroptosis) 是一种光探测器探测器可以发光.

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