两个被印记的基因被DEMETER在中央细胞中启动,并被WRKY10在内精中激活
Ke Yang1, Yuling Tang2, Yue Li3
1College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Sanya Institute of Breeding and Multiplication, Hainan University, Sanya, Hainan 572025, China; School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China; Hainan Yazhou Bay Seed Laboratory, Sanya, Hainan 572025, China.
Journal of genetics and genomics = Yi chuan xue bao
|April 10, 2024
概括
新的母体表达印记基因 (MEGs),ICF1和ICF2,调节了Arabidopsis内的发育和种子大小. 它们的激活涉及一个两步机制,预先建立了低甲基化和WRKY10转录因子结合,为印记调节提供了新的见解.
科学领域:
- 植物生殖生物学 植物生殖生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组印记是指基因组的印记.
背景情况:
- 内精的发育对于将营养物质分配给后代至关重要.
- 基因组印记,涉及父母偏向的基因表达,与内的发育有关.
- 众所周知,有限的印记基因可以调节早期内的发育和种子大小.
研究的目的:
- 为了识别和表征涉及到早期内体发育的新型印记基因在Arabidopsis thaliana.
- 阐明控制这些印制基因表达的调节机制.
- 研究这些基因在种子大小决定中的作用.
主要方法:
- 在Arabidopsis.中识别母体表达的印记基因 (MEGs).
- 对基因促进剂甲基化状态及其在基因激活中的作用的分析.
- 研究转录因子参与 (WRKY10) 在印记.
- 使用突变分析评估与种子大小相关的基因功能.
主要成果:
- 发现ICF1和ICF2作为Arabidopsis内精中的新型MEG.
- 一个两步的印记激活机制的演示,涉及预先建立的低甲基化和WRKY10.
- 母代基因通过低甲基化和WRKY10被激活,而父代基因通过CG甲基化被抑制.
- ICF基因对伊库基因突变的小种子表型有部分贡献.
结论:
- 植物印记基因激活的新型两步调节机制已被揭示.
- 传统的转录因子在激活印制基因方面发挥着重要的,以前未知的作用.
- 这一发现为植物繁殖和发育中印记的转录调节提供了新的见解.
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