相关实验视频
Updated: Aug 1, 2026
10:24
Xenopus laevis as a Model to Identify Translation Impairment
Published on: September 27, 2015
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在小鼠中,MASLD 是否在翻译中迷失了?
Aysim Gunes1, Jennifer L Estall2
1Institut de Recherches Cliniques de Montréal (IRCM), Montréal, Québec, Canada; Division of Experimental Medicine, McGill University, Montreal, Quebec, Canada.
Trends in endocrinology and metabolism: TEM
|April 10, 2024
概括
脂肪性肝病 (SLD) 的小鼠模型往往无法转化为人类患者. 诸如衰老和非食性肝脏压力因素等因素至关重要,但经常被忽视,影响研究可重现性.
科学领域:
- 肝病学 肝病学是一种肝病学.
- 翻译医学是一种翻译医学.
- 临床前研究 临床前研究
背景情况:
- 治疗脂肪性肝病 (SLD) 的药物开发面临重大挑战.
- 临床前模型对于了解疾病机制和测试治疗方法至关重要.
- 从动物模型转化为人类临床结果的不良转化是一个持续的问题.
研究的目的:
- 批判性地评估小鼠模型对于研究人类脂肪性肝病的实用性.
- 确定可能解释SLD药物开发中的翻译差距的关键变量.
- 要突出在当前临床前研究中经常被忽视的因素.
主要方法:
- 审查和讨论关于脂肪性肝病小鼠模型的现有文献.
- 在临床前模型中分析影响疾病进展和表型的因素.
- 在小鼠模型与人类SLD的实验条件的比较.
主要成果:
- 鼠标模型可能无法完全回顾人类脂肪性肝病的复杂性.
- 衰老和非饮食性肝脏压力因素是重要的混变量.
- 这些被忽视的因素可能导致临床前发现和人类疾病呈现之间的差异.
结论:
- 目前的小鼠模型需要改进,以更好地反映人类脂肪性肝病.
- 纳入诸如衰老和各种肝脏压力因素等变量对于改善翻译成功至关重要.
- 解决这些局限性可以提高SLD药物开发临床前数据的可靠性.
相关概念视频
01:31Translation
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
02:41Lethal Alleles
Agouti: A Lethal Allele
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
01:31Translation
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
02:27Nonsense-mediated mRNA Decay
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
01:19MALDI-TOF Mass Spectrometry
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...