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沙门氏菌利用膜水库入侵宿主细胞

Hongxian Zhu^1,2, Andrew M Sydor¹, Kirsten C Boddy^1,3

¹Cell Biology Program, Hospital for Sick Children, Toronto, ON, Canada.

Nature communications

|April 10, 2024

概括

沙门氏菌入侵利用先前存在的膜水库,形成细菌吸收的. 在感染期间,RAB10 GTPase活性和细菌效应剂是调动这些膜区的关键.

科学领域:

微生物学微生物学
细胞生物学细胞生物学
传染性疾病传染性疾病

背景情况:

沙门氏菌细菌使用3型分泌系统将毒性蛋白 (效应体) 注入宿主细胞.
这些效应器操纵宿主细胞过程,促进细菌进入和真空中细胞内复制.
沙门氏菌的入侵涉及到富含动氨酸的膜的形成,但膜的来源及其动员仍然不清楚.

研究的目的:

为了调查沙门氏菌引起的侵袭的膜来源.
为了阐明宿主细胞机制和细菌因素参与在入侵期间的膜动员.

主要方法:

研究了膜水库在沙门氏菌入侵中的作用.
描述了RAB10 GTPase在形成和调节膜储库中的功能.
研究了沙门氏菌效应剂从这些水库中招募出体囊子单元的情况.

主要成果:

沙门氏菌利用先前存在的膜水库,与等离子膜相连的管状隔间,形成入侵.
RAB10 GTPase活性对于这些膜储库的形成和维护至关重要.
细菌效应器 (SipC,SopE2,SopB) 从膜储存库中招募外囊子单元,以促进膜传递以供细菌吸收.

结论:

膜水库是沙门氏菌入侵的关键膜来源.
RAB10 GTPase在建立这些储库中发挥着至关重要的作用.
沙门氏菌通过效应蛋白积极劫持这些宿主细胞膜区,以促进细菌的进入.

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