在人类大脑组织中绘制ACE2和TMPRSS2联合表达:对SARS-CoV-2神经表现的含义
Tulika Gupta1, Munish Kumar2, Ujjwal Jit Kaur3
1Department of Anatomy, Postgraduate Institute of Medical Education and Research, Chandigarh, India. tulikag11@gmail.com.
Journal of neurovirology
|April 10, 2024
概括
人类大脑表达ACE2和TMPRSS2,这是SARS-CoV-2的关键入口蛋白. 这表明大脑容易感染COVID-19,这可能解释神经症状.
科学领域:
- 神经科学是一个神经科学.
- 病毒学 病毒学
- 病理学 病理学 病理学
背景情况:
- COVID-19主要影响呼吸道细胞,但也可能导致神经问题.
- SARS-CoV-2 使用 ACE2 和 TMPRSS2 进入细胞.
- 关于超出嗅觉区域的人类大脑组织中这些受体的数据有限.
研究的目的:
- 为了绘制 ACE2 和 TMPRSS2.2. 在整个大脑中的表达.
- 为了研究病毒进入蛋白质表达的与年龄相关的变化.
- 评估正常人脑组织对SARS-CoV-2的敏感性.
主要方法:
- 在人脑组织样本中分析基因表达 (qRT-PCR) 和蛋白质共表达 (免疫光).
- 来自大脑皮质,海马体,基底,丘脑,下丘脑,大脑干和小脑的样本.
- 组织分为两个年龄组:年龄在40岁以下和40岁以上.
主要成果:
- 分别在下丘脑和丘脑中,ACE2和TMPRSS2基因表达最高.
- 在所有被检查的大脑区域中检测到ACE2和TMPRSS2蛋白质.
- 在下丘脑中观察到ACE2和TMPRSS2的最大同定位.
- 没有发现与年龄相关的显着表达趋势.
结论:
- 正常的人类大脑组织表达了SARS-CoV-2进入所需的受体.
- 整个大脑中ACE2和TMPRSS2的存在支持潜在的病毒易感性.
- 这种广泛的表达可能导致COVID-19患者的神经和认知障碍.
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