提高简单疹角膜炎的聚合酶连锁反应诊断率:试点研究结果
Alice L Milligan1, Jeremy J Hoffman1,2, Yan Ning Neo1
1Corneal and External Diseases Department and Accident and Emergency Department, Moorfields Eye Hospital NHS Foundation Trust, London, United Kingdom.
Digital journal of ophthalmology : DJO
|April 11, 2024
概括
改善单纯疹病毒 (HSV) 角质炎诊断,教育干预显著提高了聚合酶链反应 (PCR) 灵敏度. 适当的抽样技术可以提高眼部感染的PCR准确性.
科学领域:
- 眼科医生 眼科 眼科
- 传染性疾病 传染性疾病
- 分子诊断学 分子诊断学
背景情况:
- 简单疹病毒 (HSV) 角膜炎的诊断依赖于实验室确认.
- 聚合酶连锁反应 (PCR) 对HSV角质炎的敏感性在历史上较低 (25%),而不是粘膜皮肤疾病 (75%).
研究的目的:
- 为了提高对HSV角质炎的PCR的诊断产量.
- 通过教育干预以扫描技术为重点,提高PCR敏感性.
主要方法:
- 在伦敦的一家第三级眼科转诊医院对炎病例的回顾性审查.
- 诊断数据的比较,从之前和之后的时间段,在教育计划的扫描技术.
主要成果:
- 干预后观察到实验室确认的HSV-1诊断的显著增加,无论是新发病 (11.1%至57.7%) 还是复发病例 (20%至57.6%).
- 在具有上皮缺陷的眼睛中,阳性HSV-1 PCR率在教育干预后从19%上升到62%.
- 在3%的病例中检测到疹病毒DNA,并确定了一例Acanthamoeba角膜炎.
结论:
- 适当的抽取技术可以显著提高PCR用于诊断HSV角质炎的灵敏度.
- 教育干预表明PCR有可能成为比以前报告的眼部HSV感染更敏感的诊断工具.
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