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低温等离子体喷射抑制细菌殖民,并通过反应性物种影响伤口愈合

Yang Ge¹, Jun Wang^1,2, DongHua Gu³

¹The CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China.

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society

|April 11, 2024

概括

低温等离子喷射有效治疗慢性黄金菌感染的伤口. 中等剂量的血通过减少细菌和炎症促进愈合,同时增强组织修复.

科学领域:

生物医学工程生物医学工程
等离子体物理学的物理学
伤口治愈研究研究伤口治愈研究

背景情况:

由于持续的细菌感染,特别是黄金葡萄球菌感染,慢性伤口存在重大挑战.
目前的治疗方法经常面临有效性的局限性,并可能导致并发症.
低温等离子喷射器 (LTPJs) 提供了一种具有抗菌性能的新型治疗方法.

研究的目的:

在小鼠模型中研究基于的LTPJ在治疗慢性金黄色葡萄球菌感染的伤口中的有效性.
确定最佳的血参数,最大限度地提高抗菌效果,同时最大限度地减少潜在的组织损伤.
阐明LTPJ促进伤口愈合的潜在机制.

主要方法:

物理化学分析和体外实验以将血参数与活性和氧物种 (RNOS) 含量和抗菌能力相关联.
优化LTPJ参数 (流量和功率) 用于治疗黄菌感染的伤口.
在小鼠体内研究,以评估中剂量LTPJ对细菌负载,炎症,细胞外基质沉积和生长因子水平的影响.

主要成果:

最佳的LTPJ参数被确定为每分钟4标准升和35W,与等离子激活溶液也表现出杀菌作用.
中剂量的LTPJ抑制了细菌生长,并在早期伤口愈合阶段促进了矩阵金属蛋白酶-9 (MMP-9) 的表达.
经过LTPJ治疗,原体沉积增加,炎症减少,恢复血管密度,并在后期阶段使TGF-β水平正常化.

结论:

中剂量LTPJ有利于慢性伤口恢复,平衡抗菌作用与组织再生.
LTPJ显示出作为治疗工具的潜力,用于治疗慢性,细菌感染的伤口.
精心选择血剂量对于优化伤口愈合结果至关重要.

关键词:

在RNS RNS RNS中使用.这就是ROSOS ROS.它是一种抗菌药物,具有抗菌作用.慢性感染的伤口感染.低温等离子体低温等离子体

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