相关实验视频

Updated: Jun 28, 2025

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

中介细胞干细胞衍生-外生体增强的羊水膜提取物促进角膜角质细胞增殖

Fatma Zehra Erkoc-Biradli¹, Berkay Erenay¹, Alp Ozgun²

¹Biomimetics and Bioinspired Biomaterials Research Laboratory, Institute of Biomedical Engineering, Bogaziçi University, Istanbul, Turkey.

Biotechnology progress

|April 11, 2024

概括

结合羊水膜提取物 (AME) 和华顿果介质干细胞衍生的外体细胞 (WJ-MSC-Exos),显著促进角膜角细胞的增殖和伤口愈合. 这种协同方法对先进的角膜修复策略有前途.

科学领域:

再生医学是一种再生医学.
组织工程是组织工程.
眼科医生眼科眼科

背景情况:

胚胎膜提取物 (AME) 和沃顿的果介质干细胞衍生的外体细胞 (WJ-MSC-Exos) 已因其在伤口愈合中的治疗潜力而得到认可.
虽然单独研究,但AME和WJ-MSC-Exos在角膜应用中的协同效应仍未得到充分研究.

研究的目的:

调查AME和WJ-MSC-Exos对角膜角质细胞增殖和迁移的联合影响.
评估AME和WJ-MSC-Exos对体外伤口愈合和细胞形态的协同影响.

主要方法:

角膜角细胞被用AME,WJ-MSC-Exos或两者的组合治疗.
进行了细胞增殖测试,以评估细胞随时间的增长.
实验室内伤口愈合模型被用来测量细胞迁移和关闭率.

主要成果:

与单独的AME相比,AME (50μg/mL) 和WJ-MSC-Exos (3 × 10^5 Exo/mL) 的组合协同增强了角膜角质细胞的增殖.
与单独使用WJ-MSC-Exos相比,混合处理显著提高了伤口关闭率.
联合治疗促进了角膜角质细胞增强的增殖和伤口愈合倾向.

结论:

AME和WJ-MSC-Exos的协同组合为角膜伤口愈合提供了一个有前途的治疗策略.
这种综合方法可以显著改善角膜角质细胞的增殖和迁移.
对AME和WJ-MSC-Exos混合物的进一步研究可以为新的角膜修复疗法奠定基础.

关键词:

沃顿的果介质干细胞来源于外生体.胚胎膜提取物胚胎膜提取物角膜角质细胞细胞.在实验室中,角膜伤口愈合.

更多相关视频

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Published on: October 27, 2023

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

Published on: February 6, 2020

相关概念视频

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...