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用光分子旋转器染料和核酸污染剂标记化细胞表面的甘氨酸

Alen Koçak¹, Amal K Homer¹, Antonia Feida¹

¹Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489 Berlin, Germany. oliver.seitz@chemie.hu-berlin.de.

Chemical communications (Cambridge, England)

|April 11, 2024

概括

用光分子旋转器 (FMR) 在活细胞上对酸进行共价标记,可实现无洗的细胞表面成像. 使用FMR和不敏感染料的双重标记可以检测细胞结构的变化,如交叉链接.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
光显微镜光显微镜

背景情况:

酸是关键的细胞表面甘氨酸,参与各种生物过程.
目前用于细胞表面成像的方法通常需要多个洗步骤,这可能是繁的,并影响细胞活力.
光分子转子 (FMR) 对其局部环境敏感,为新型成像应用提供了潜力.

研究的目的:

开发一种无洗的方法,用于对活细胞表面的酸成像.
利用FMRs的环境灵敏度来检测细胞变化.
探索用于生物研究的酸与FMR的共价标记的潜力.

主要方法:

在活细胞表面和使用FMRs的粘素上对酸的共价标记 (CCVJ,Cy3,酸).
使用光显微镜对标记细胞进行成像.
双重标签实验将FMR与环境不敏感的染料结合起来.

主要成果:

用FMR对酸的共价标记显著增加了它们的光.
这种标记使得活细胞表面的有效,无洗成像成为可能.
双重标签允许检测变化,例如交叉链接的变化.

结论:

用FMR对酸进行共价标记,为活细胞表面成像提供了强大而高效的方法.
这种方法通过消除洗步骤来简化细胞成像协议.
该技术对研究细胞表面结构和功能的动态变化具有前景.

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