新生儿肠杆菌吸收人乳养婴儿消化液中的细胞外囊泡
Claire Yung1, Yang Zhang1, Madeline Kuhn1
1Department of Pediatrics, Pediatric GI Division, School of Medicine, Oregon Health and Science University, Portland, Oregon, USA.
Journal of extracellular vesicles
|April 11, 2024
概括
来自母乳的细胞外囊泡 (EVs) 能够在新生儿消化过程中存活下来,并被肠道细胞吸收. 这一发现支持牛奶EV在婴儿营养和治疗中的潜在用途.
科学领域:
- 新生儿营养新生儿营养
- 细胞外囊泡生物学 细胞外囊泡生物学
- 胃肠病学 胃肠病学
背景情况:
- 人类牛奶细胞外囊泡 (HMEVs) 显示出对肠道健康的临床前前景.
- 在新生儿消化过程中,HMEVs的生存和吸收仍然没有特征.
- 这种知识缺口限制了HMEV货物的治疗和营养应用.
研究的目的:
- 开发一种管道,从母乳和新生儿消化器中分离细胞外囊泡 (EVs).
- 测试HMEVs在体内新生儿消化中存活并被肠上皮细胞 (IECs) 内化的假设.
主要方法:
- 使用密度梯度超离心,过和沉,从母乳和新生儿消化器中分离EV.
- 通过电子显微镜,西欧涂抹,纳米粒子跟踪分析和显微镜进行EV特征.
- 使用人类新生儿体的EV吸收研究.
主要成果:
- 从母乳和新生儿消化器 (dEVs) 中成功分离了EVs.
- 孤立的EVs表现出特有的形态和表面标记 (CD81,CD9),以及像β-素这样的牛奶蛋白质的枯竭.
- 新生儿肠杆菌体表现出dEVs的快速吸收,部分是通过克拉斯林介导的内细胞分裂.
结论:
- 细胞外囊泡可以有效地从新生儿消化液中分离出来.
- 来自消化器的EV被新生儿肠道上皮细胞吸收.
- 这些发现表明乳制品EVs在新生儿肠道健康和发育中的潜在作用.
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