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Updated: Jun 28, 2025

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
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Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

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开发和评估一个单管嵌套PCR与色度测定对Mycobacterium结核病检测的测定

Marcela Pereira Salazar1,2, Juliana Figueiredo da Costa Lima Suassuna Monteiro1,2, Wlisses Henrique Veloso Carvalho-Silva1

  • 1Laboratory of Immunoepidemiology, Department of Immunology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Pernambuco, 50740-465, Brazil.

BioTechniques
|April 11, 2024
PubMed
概括

这项研究开发了一种新的色度测量单管嵌套PCR (STNPCR) 用于检测Mycobacterium结核病 (Mtb) DNA. 该方法显示检测极限为1ppg/μl,但具有中度的灵敏度和特异性.

关键词:
在IS6110中使用.Mtb复合体是一个复合体.这是STNPCR.快速分子测试 快速分子测试结核病的诊断 结核病的诊断

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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