高速原子力显微镜揭示了无尾核子体的核细胞滑动和DNA解封/包装动态
Shin Morioka1, Takumi Oishi2,3, Suguru Hatazawa2
1Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan.
Nano letters
|April 11, 2024
概括
基因组尾巴稳定细胞核,影响基因表达. 删除这些尾巴大大增加了核酶体动力学,揭示了它们在DNA相互作用和表观遗传调节中的关键作用.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 生物物理学的生物物理.
背景情况:
- 核,DNA包装的基本单元,含有具有必要的N-终端尾巴的基因组蛋白.
- 这些组织蛋白尾巴对于通过后翻译修饰 (PTMs) 进行表观遗传基因调节至关重要.
- 基因组尾对单分子核体动态的影响尚未完全理解.
研究的目的:
- 为了研究N-终端组素尾在单个分子水平的核体动力学中的作用.
- 为了确定单个或所有基因组尾巴的缺失如何影响核细胞结构和稳定性.
- 阐明基因组尾对基因组-DNA相互作用的贡献.
主要方法:
- 高速原子力显微镜 (HS-AFM) 用于可视化核体动力学.
- 实验对缺少个体基因组N端尾或所有N端尾的核体进行了实验.
- 分析了核细胞滑动,DNA解包/包装以及结构参数.
主要成果:
- 删除所有N端尾部导致DNA的6.7个基对从基质核中脱离.
- 与野生类型相比,没有尾巴的核细胞的DNA入出口角增加了18°.
- 缺乏H2B和H3尾巴的核细胞在0.3秒内显示出动力 (滑动,解/包装) 增加了10倍.
结论:
- N-终端的组素尾在稳定核酶体结构方面发挥着重要作用.
- 基因组尾巴是核酶体动力学和基因组-DNA相互作用的关键调节者.
- 基因细胞尾部的翻译后修改可能会调节核体动力学,影响基因表达.
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