相关实验视频

Updated: Jul 15, 2026

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

Published on: August 12, 2016

在循环单细胞中MAFB基因的表达与T2DM患者的慢性炎症状况有关

Wanliang Zhang¹, Wenyun Chen¹, Jingwei Lei¹

¹The Key Laboratory of Laboratory Medical Diagnostics in the Ministry of Education and Department of Clinical Biochemistry, College of Laboratory Medicine, Chongqing Medical University, Chongqing, China.

|April 11, 2024

概括

单细胞中MAFB基因表达在2型糖尿病 (T2DM) 中升高,与胰岛素抵抗和炎症相关. 这种生物标志物有助于预测和诊断T2DM.

科学领域:

免疫学免疫学免疫学
内分泌学在内分泌学.
遗传学是一个遗传学.

背景情况:

免疫细胞介导的慢性炎症有助于2型糖尿病 (T2DM).
识别循环免疫细胞中的炎症标志物对于预测胰岛素抵抗 (IR) 和T2DM发展至关重要.

研究的目的:

在外周血液单核细胞 (PBMCs) 中识别新的炎症生物标志物,用于T2DM预测.
调查MAFB基因表达在T2DM病原体中的作用及其与IR的关联.

主要方法:

大量转录组测序 (大量RNA-seq) 和单细胞RNA测序 (scRNA-seq) 的PBMCs.
基因整合分析,接收器操作特征 (ROC) 曲线分析和基于人口的队列研究.

主要成果:

在T2DM患者的PBMC中,MAFB基因表达显著上调,与HOMA-IR和炎症标志物相关.
MAFB证明了T2DM的预测价值,主要在非经典单细胞中观察到表达的增加.

结论:

在循环单细胞中MAFB表达的增加可能表明T2DM的慢性炎症.
MAFB作为一个潜在的临床生物标志物用于预测和诊断T2DM.

关键词:

在MAFBB中,MAFBB是MAFB.这是PBMCs.这就是T2DM.慢性炎症是一种慢性炎症.

更多相关视频

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Published on: October 17, 2017

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Published on: August 12, 2019

相关概念视频

Chronic Inflammation: Introduction

Chronic Inflammation: Introduction

Chronic inflammation is a prolonged, dysregulated immune response that persists for weeks to years when the inciting stimulus is difficult to eradicate or when self‑antigens drive ongoing reactivity. Morphologically, it is defined by mononuclear cell infiltration, progressive tissue destruction, and concurrent attempts at healing via angiogenesis and fibrosis. Compared with acute inflammation, edema is less prominent while cellular infiltration predominates; triggers include persistent...