拉曼差异光谱和U-Net卷积神经网络用于皮肤神经纤维瘤的分子分析
Levi Matthies1,2, Hendrik Amir-Kabirian1, Medhanie T Gebrekidan3
1Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
PloS one
|April 11, 2024
概括
这项研究引入了拉曼光谱学和人工智能,用于对神经纤维瘤1型神经纤维瘤 (NF1) 的皮肤神经纤维瘤的客观实时诊断. 这种非侵入性光学技术有助于精确的手术切除,改善患者的治疗结果.
科学领域:
- 生物医学光学 生物医学光学
- 人工智能在医学中的应用
- 在瘤学瘤学.
背景情况:
- 在神经纤维素瘤类型1 (NF1) 中的皮肤神经纤维瘤会导致显著的美学,疼痛和功能问题.
- 目前的手术切除依赖于主观边缘评估,导致不完全切除和复发.
- 需要客观的实时诊断工具来指导外科切除和改善患者护理.
研究的目的:
- 引入转移刺激拉曼光谱 (SERS) 与深度神经网络 (DNN) 结合,以客观,实时诊断皮肤神经纤维瘤.
- 评估这种技术在分辨神经纤维瘤和相邻的健康组织中的有效性.
- 在NF1患者中为指导性外科切除提供基础.
主要方法:
- 34个外科病理样本的活体分析 (9个皮肤神经纤维瘤,25个生理组织).
- 移动刺激拉曼光谱 (SERS) 的应用用于光谱数据采集.
- 深度神经网络 (U-Net) 用于光谱处理,结合主要组件和线性差异分析进行分类.
主要成果:
- 在区分皮肤神经纤维瘤与生理组织方面具有高的诊断性能.
- 获得了100%的灵敏度,97.3%的特异性和97.6%的整体分类准确度.
- 成功地将光谱峰与它们各自的分子起源分配,验证了该技术.
结论:
- 带有人工智能的转移刺激拉曼光谱 (SERS) 为诊断皮肤神经纤维瘤提供了一种有前途的非侵入性方法.
- 这种光学技术有可能显著改善NF1的诊断和手术治疗.
- 客观的实时反可以指导外科医生,最大限度地减少残留瘤和复发率.
相关概念视频
01:20Raman Spectroscopy: Overview
373
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
373
01:26Raman Spectroscopy Instrumentation: Overview
332
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
332