通过Maitake提取物对新重建的人体肠道微生物群的积极调节,在体外有助于增强肠道环境
Alessandra De Giani1, Federica Perillo2, Alberto Baeri1
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milano, Italy.
PloS one
|April 11, 2024
概括
这项研究重建了最小的肠道微生物群,以调查梅提取物和益生菌的影响. 该组合积极调节细菌群落,产生有益的代谢物,促进抗氧化和抗炎作用.
科学领域:
- 微生物学和肠道健康
- 代谢学 代谢学 代谢学
- 免疫学 免疫学 免疫学
背景情况:
- 人的肠道微生物群对健康至关重要,涉及复杂的微生物-微生物相互作用.
- 了解这个生态系统需要简化,代表性模型.
- 关键的科目包括Bacillota,Bacteroidota,Pseudomonadota和Actinomycetota. 这些科目包括:
研究的目的:
- 为了在体外重建一个最小的人类肠道微生物群.
- 评估Maitake提取物 (益生菌) 和益生菌配方的协同作用.
- 了解细菌群落的调节,代谢物生产和宿主健康影响.
主要方法:
- 一个最小的肠道微生物群模型的重建.
- 使用梅竹提取物和益生菌混合物 (Lactobacillus和Bifidobacterium).
- 定量PCR (qPCR) 用于细菌菌株分析,气相色谱-质谱 (GC-MSD) 用于代谢物概况,以及人类细胞系测定宿主影响.
主要成果:
- 在体外细菌群体的积极调节,增加了乳球菌植物菌和生物细菌动物亚种的水平. 在48小时后的乳糖.
- 产生有益的代谢物,包括乳酸盐,丁酸盐和酸盐.
- 已证明抗氧化作用 (HT-29细胞中的SOD1,NQO1上调) 和抗炎作用 (PBMC中的IL-10产生).
结论:
- 益生菌和Maitake提取物的协同管理积极调节肠道微生物群的组成和功能.
- 这种组合促进了具有有益健康影响的耐受性微环境.
- 在体外最小肠道微生物群模型对于研究宿主-微生物相互作用是有效的.
相关概念视频
Probiotics
299
Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
299
Introduction to the Human Microbiota
222
Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity,...
222
Development of Human Microbiota
68
The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from...
68
Microbiota of the Stomach and Small Intestine
92
The human gastrointestinal (GI) tract is characterized by distinct physicochemical conditions that shape its microbial communities. Among these, the stomach presents a particularly challenging environment for microbial colonization due to its highly acidic pH, ranging from 1 to 3. This extreme acidity effectively limits microbial density. However, certain acid-tolerant microorganisms are capable of surviving in this niche. Notably, Helicobacter pylori can colonize the gastric mucosa,...
92
Microbiota of the Large Intestine
108
The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...
108
Functions of the Gut Microbiota
254
The gut microbiota includes trillions of microorganisms that colonize the human gastrointestinal tract, including bacteria, archaea, viruses, and fungi. This complex ecosystem plays a critical role in maintaining intestinal and systemic health. Most of these microbes inhabit the large intestine, establishing a relatively stable and diverse community that contributes to gut homeostasis through various metabolic, immunological, and protective mechanisms.Dominant bacterial phyla, such as...
254


