空气中的抗生素耐药和微生物在制药工厂中的微生物组
Hong Bai1, Liang-Ying He1, Fang-Zhou Gao1
1SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, China.
Environment international
|April 11, 2024
概括
空气传播从制药工厂传播抗生素耐药性基因 (ARG),主要来源是土壤和废水. 污水处理区的ARG水平较高,增加了水平基因转移风险.
科学领域:
- 环境微生物学环境微生物学
- 公共卫生 公共卫生
- 基因组学就是基因组学.
背景情况:
- 抗菌素耐药性 (AMR) 是全球主要的公共卫生威胁.
- 抗生素耐药性基因 (ARG) 的空气传播是一种被低估的环境传播途径.
- 制药工厂被确定为ARG和抗生素耐药细菌 (ARB) 的重要来源.
研究的目的:
- 在中国的三个制药工厂内调查空气中的微生物群和抗体.
- 确定来自这些工厂的空中ARG的来源和特征.
- 评估与空中ARG相关的水平基因转移和环境风险的潜力.
主要方法:
- 使用元基因组测序来分析空气中的微生物群落及其相关的ARG.
- 从三个制药工厂环境中采集了空气样本.
- 分析包括识别ARG来源,量化ARG丰度,评估等离子体关联和病毒存在.
主要成果:
- 土壤和废水是空气中抗体的主要贡献者 (分别为38.45%和28.53%).
- 在空气样本中检测到具有临床意义的ARG (例如,vanR/vanS,blaOXA,CfxA).
- 污水处理区的ARG相对丰度显著高 (0.64个副本/16SrRNA) 和等离子体协会 (约. 50%) 的比例.
- 在废水处理区的空气中观察到高丰度的菌体 (2451.70 PPM).
- 确定了1,043个病毒操作分类单元,其中存在各种CRISPR-Cas系统.
结论:
- 制药工厂对空气中的ARG做出了重大贡献,土壤和废水是主要来源.
- 空气中的ARG,特别是在废水处理区,由于高丰度和等离子体协会,造成环境风险.
- 病毒和CRISPR-Cas系统的存在表明活跃的水平基因转移,可能放大AMR传播.
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