一种新型禽类腺病毒8b菌株的鉴定,致病性和分子特征
Yapeng Song1, Lin Liu1, Wenjie Sun1
1College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.
Poultry science
|April 11, 2024
概括
一种新的禽类腺病毒 (FAdV) 菌株,HN1472,被确定为FAdV-8a和FAdV-8b的复合物,导致严重的包容体肝炎 (IBH) 和的高死亡率. 这项研究详细介绍了它的遗传构成和病原性.
科学领域:
- 兽医病毒学 兽医病毒学
- 禽类病理学 禽类病理学
- 分子流行病学分子流行病学
背景情况:
- 自2012年以来,包容体肝炎 (IBH) 的发病率在全球范围内有所上升,导致家禽业的重大损失.
- 高致病性禽类腺病毒 (FAdVs) 与IBH疫情有关.
- 了解FAdV遗传多样性和病原性对于疾病控制至关重要.
研究的目的:
- 调查从IBH受影响的群体中分离的高度致病性FAdV菌株 (HN1472) 的遗传特征,遗传突变和致病性.
- 为了确定FAdV HN1472菌株的起源和遗传构成.
- 评估FAdV HN1472菌株在中的毒性和组织分布.
主要方法:
- 从受影响的家禽中隔离和识别FAdV菌株HN1472.
- 用赫克逊基因,纤维基因和ORF19基因进行了遗传学和重组分析.
- 在1天大的无特定病原体 (SPF) 中进行致病性测试,包括死亡率评估和临床迹象观察.
- 在挑战后3天进行组织分布分析.
主要成果:
- 证实FAdV HN1472是FAdV-8a和FAdV-8b的重组菌株,显示关键基因的遗传差异.
- 在SPF中,HN1472引发了严重的IBH,导致80%的死亡率和特征性临床症状.
- 在多种组织中检测到复合FAdV血清型8b (FAdV-8b),在肝脏和胃肠中度高.
结论:
- FAdV HN1472代表了在中国流传的高度致病性复合FAdV-8b菌株.
- 遗传重组有助于FAdV血清型8b的进化和致病性.
- 这些发现有助于更好地了解FAdV-8b的流行病学和病原性,有助于制定控制战略.
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