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在Aquilaria sinensis中的III型多基基合成酶基因家族的分子进化和特征

Xupo Ding¹, Hao Wang¹, Shengzhuo Huang¹

¹Key Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China; International Joint Research Center of Agarwood, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China; Hainan Engineering Research Center of Agarwood, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China.

Plant physiology and biochemistry : PPB

|April 11, 2024

概括

两种新型的III型多基基合成酶 (PKSs),AsPKS07和AsPKS08,已被确定,并被证明可以促进阿基拉里亚树 (Aquilaria sinensis) 中的2-(2-乙烯) 染色体 (PEC) 生物合成,从而提供了对木形成的见解.

科学领域:

植物生物化学植物生物化学
分子进化是分子进化的过程.
代谢工程是代谢工程.

背景情况:

2 - 乙基) 染色体 (PEC) 衍生物是阿加木的形成和质量的关键标志物.
PECs的生物合成和调节途径在很大程度上是未知的.
第三种类型的多基合成酶 (PKSs) 参与了 Aquilaria sinensis 中的 PEC 生物合成,但它们的进化动态研究不足.

研究的目的:

为了全面分析PKS基因在Aquilaria sinensis的演变.
描述参与PEC生物合成的新型PKS基因.
调查控制PEC生产的监管机制.

主要方法:

在12个植物基因组中对PKS基因进行了植物遗传学分析.
在Nicotiana benthamiana中选择AsPKS基因的基因表达分析.
促进体分析以确定与转录因子的相互作用.

主要成果:

通过进化分析确定了AsPKS基因的一个独特分支,包括AsPKS01,AsPKS07和AsPKS08.
在N. benthamiana中AsPKS07和AsPKS08的短暂表达导致检测到PECs.
AsPKS07/AsPKS08促进体和S亚家族bZIP转录因子之间的相互作用表明监管控制.

结论:

AsPKS07和AsPKS08积极促进PEC生物合成,为这种途径提供了新的理解.
鉴定的转录因子相互作用为PEC生产的调节提供了洞察力.
这些发现为生物工程战略奠定了基础,以提高PEC和阿加木的生产.

关键词:

2-(2-甲基) 染色体生物合成阿加拉木是阿加拉木的一种木材.进化进化进化进化进化进化进化聚基化合成酶的作用过渡式的表达式是暂时的.

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