相关实验视频
Updated: Jun 28, 2025

09:20
CRISPR-Mediated Reorganization of Chromatin Loop Structure
Published on: September 14, 2018
12.6K
SAFB限制了与L1仿真转录相关的接触域边界
Yaqiang Hong1, Luyao Bie1, Tao Zhang1
1Tsinghua University-Peking University Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Molecular cell
|April 11, 2024
概括
长间隔的元素-1 (LINE-1或L1) 逆转移子丰富RNA聚合酶II (RNA Pol II) 并形成基因组域边界. 脚手架附着因子B (SAFB) 抑制了这种L1活动,以维持基因调节.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 长间隔的元素-1 (LINE-1或L1) 逆转移体构成了人类基因组的一个重要部分.
- 已知L1元素会产生遗传变异,并可能导致疾病.
- 精确的监管机制和L1元素的功能作用在很大程度上仍未被阐明.
研究的目的:
- 研究L1元素在基因组组织和调节中的作用.
- 确定调节L1活动及其对基因组的影响的因素.
- 了解L1介导的基因组变化的功能后果.
主要方法:
- 在L1位点对RNA聚合酶II (RNA Pol II) 丰富的分析.
- 检测和表征L1仿真转录.
- 通过细胞测试评估L1在形成接触域边界中的作用.
- 研究L1转录和支架附着因子B (SAFB) 之间的相互作用.
- 功能性研究涉及急性抑制RNA Pol II转录和L1删除.
主要成果:
- L1元素丰富RNA Pol II,表达化学转录,并在人体细胞中建立接触域边界.
- 脚手架附着因子B (SAFB) 与L1转录结合,抑制RNA Pol II丰富和L1的影响.
- 抑制RNA Pol II转录破坏了L1关联域边界,证实了转录依赖的机制.
- L1删除会损害域边界的形成,而L1插入则有助于特定物种的边界.
结论:
- 通过创建RNA Pol II丰富区域和改变域结构,L1元素积极塑造基因组组织.
- SAFB作为一个关键的调节器,调节L1和RNA Pol II活动,以保持适当的基因调节.
- L1对基因组组织的影响依赖于转录,并且已经演变为产生特定物种的基因组特征.
相关概念视频
Cooperative Binding of Transcription Regulators
6.4K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.4K
Eukaryotic Transcription Inhibitors
9.8K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
9.8K
Conserved Binding Sites
4.2K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.2K
Cis-regulatory Sequences
9.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.9K
Non-LTR Retrotransposons
11.5K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.5K
Conservative Site-specific Recombination and Phase Variation
6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.0K

