简单的Anisakis (s. s.) 对杀虫剂的反应 - - 对外来生物代谢过程的具体变化
Robert Stryiński1, Iwona Polak1, Anna Gawryluk1
1Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A Str., 10-719, Olsztyn, Poland.
Experimental parasitology
|April 11, 2024
概括
这项研究探讨了Anisakis simplex (s.s.) 幼虫如何对抗虫药物ivermectin和pyrantel的反应. 这两种药物都改变了基因表达和氧化状态,这表明它们与对抗这种寄生虫的有效性有关.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 药物发现 药物发现 药物发现
背景情况:
- 阿尼萨基病是一种由简单的阿尼萨基 (sensu stricto) 和Anisakis pegreffii幼虫引起的食物传播的寄生动物性 zoonosis.
- 污染的生或未煮熟的海鲜消费是主要的传播途径.
- 对西病的有效治疗方法有限,需要对新型治疗剂进行研究.
研究的目的:
- 在体外研究抗虫药物伊弗梅克丁 (IVM) 和皮兰特尔 (PYR) 对Anisakis simplex (s. s.) 幼虫的作用.
- 分析IVM和PYR对A.simplex (s.s.) 的基因表达和氧化状态的影响.
主要方法:
- 来自波罗的海鱼的Anisakis simplex (s.s.) 幼虫 (L3和L4阶段) 在体外培养.
- 在无氧条件下,幼虫在不同时间内暴露于不同度的IVM或PYR.
- 分析了基因表达 (动氨酸,ABC载体,抗氧化酶,神经递质受体) 和氧化状态 (TAC,GST活性).
主要成果:
- 皮兰 (PYR) 显著降低了L3阶段A.simplex (s.s.) 幼虫的移动性,与ivermectin (IVM) 相比.
- 两种IVM和PYR治疗都改变了ABC载体和抗氧化酶的mRNA水平.
- 在接受IVM和PYR治疗的幼虫中,总抗氧化能力 (TAC) 和谷氨S转移酶 (GST) 活性增加.
结论:
- 在IVM和PYR的反应中,Anisakis simplex (s.s.) 呈现出改变的基因表达,特别是在ABC载体和抗氧化剂通路中.
- 观察到的基因表达和氧化状态的变化表明了这些药物的虫杀伤活性的潜在机制.
- 需要进一步的研究,以探索IVM和PYR对抗anisakiasis的治疗潜力.
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