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同时有效地溶解和结构修改花:针对细菌菌的增殖

Wenying Wang¹, Lianzhu Lin¹, Mouming Zhao¹

¹School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou 510641, China.

International journal of biological macromolecules

|April 11, 2024

概括

这项研究开发了一种绿色的酶策略,从花 morifolium Ramat.中提取RG-I丰富的pectin.

科学领域:

食品科学食品科学食品科学
生物技术是生物技术.
植物生物化学植物生物化学

背景情况:

来自金 morifolium Ramat. 的性多糖化物. "Hangbaiju" (CMH) 具有生物活性特性.
高水平的homogalacturonan (HG) 域含量限制了CMH乳腺素的前生物潜力.
需要新的提取方法来增强CMHpectin的益生菌疗效.

研究的目的:

开发一个高效的和绿色的取策略,从CMH. 益生菌培.
为了增强CMH乳腺素的结构特征和益生菌活性.
调查细胞酶和pectinase对pectin提取和属性的协同效应.

主要方法:

通过热水,细胞酶辅助,中温和深性溶剂方法从CMH中提取pectin.
优化酶提取使用细胞酶和pectinase的组合.
蛋白结构的表征,包括HG和RG-I域的比例.
通过测量 Bacteroides 和混合益生菌的增殖来评估益生菌活性.

主要成果:

使用细胞酶和点酶 (3:1比) 的酶提取产生了最佳的CMH点 (CMHP),产量高 (15.15%).
最佳的CMHP表现出增强的细菌菌种繁殖活性,超过了胰岛素.
结构分析显示,RG-I域较高 (80.30%),HG域中度较高,在最佳CMHP中分支增加.

结论:

一个绿色的,协同作用的酶策略有效地从CMH中提取RG-I丰富的益生菌pectin.
优化的CMHP显示出优越的益生菌潜力,与传统的益生菌如inulin相比.
这种方法提供了一种可持续的方法,用于从植物来源开发功能性食品成分.

关键词:

细胞酶和点酶的组合.提取提取提取修改修改修改是一个质素 (Pectin) 是一种可怕的物质.益生菌活动的前生物.结构领域结构领域

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