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人类肌结的工程化体外模型

Mitchell Josvai¹, Erzsebet Polyak², Meghana Kalluri¹

¹Department of Biomedical Engineering, University of Wisconsin-Madison, Engineering Centers Building, 2126, 1550 Engineering Dr, Madison WI 53706, USA; Wisconsin Institute for Discovery, University of Wisconsin-Madison, 330 N Orchard St, Madison, WI 53715, USA.

Acta biomaterialia

|April 11, 2024

概括

这项研究使用人类细胞开发了一种新的肌结 (MTJ) 试管模型. 该模型成功地复制了MTJ的结构和功能,使力传递研究成为可能.

科学领域:

生物医学工程生物医学工程
细胞生物学细胞生物学
组织工程是组织工程.

背景情况:

肌关节 (MTJ) 对于肌肉和肌之间的力传递至关重要,但容易受伤.
现有的体外模型缺乏本地MTJ的结构和机械忠实性.
需要准确的模型来研究MTJ发育,损伤和疾病.

研究的目的:

开发一个新的2D体外模型的人类肌结 (MTJ).
为了研究肌细胞 - 肌细胞相互作用和机械合的形成.
为研究MTJ发育,损伤和病理学创建一个平台.

主要方法:

人类胚胎干细胞衍生的肌细胞和初级肌细胞的共同培养,使用微型ECM和2井室.
空间限制培养以指导细胞组织和结节形成.
免疫细胞化学评估MTJ标记物表达和细胞对齐.
机械刺激和应变分析以评估力转导.

主要成果:

工程MTJ连接显示了肌细胞-肌细胞间的数字化和增强的细胞组织.
观察到关键MTJ标记物的差异表达.
电刺激的肌细胞表现出收缩性应变,传递给被动的肌细胞,模仿本地MTJ力转导.
没有图案的细胞显示组织和协调受损.

结论:

开发的平台成功地模拟了人类MTJ形成和机械合.
这种体外系统复制了在肌细胞-肌细胞界面上的本地MTJ力转导.
该模型为研究人类MTJ生物学,损伤和治疗策略提供了有价值的工具.

关键词:

在体外建模模型.微型模式微型模式肌结节的结节肌结节骨肌细胞骨肌细胞青细胞青细胞青细胞

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