通过细胞壁完整性MAPK途径,有效的酵母适应性转录反应需要mRNA解剖激活器Pat1
Verónica Pulido1, Jose M Rodríguez-Peña1, Graciela Alonso1
1Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Instituto Ramón y Cajal de Investigaciones Sanitarias (IRYCIS), 28040 Madrid, Spain.
Journal of molecular biology
|April 11, 2024
概括
Pat1是一种脱皮激活剂,在酵母菌中起着至关重要的作用.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 基因规则 基因规则
背景情况:
- 细胞mRNA水平由转录和降解来调节.
- 5'-3'mRNA降解途径涉及可以结合染色素的因素.
- 像Pat1这样的脱皮激活器是这个途径的关键组成部分.
研究的目的:
- 研究Pat1在酵母对细胞壁应激的适应性转录反应中的作用.
- 为了确定Pat1是否影响基因转录或压力下的mRNA稳定性.
主要方法:
- 研究了Pat1在酵母适应性转录反应中的作用.
- 分析了细胞壁完整性MAPK通路基因的转录诱导.
- 评估了mRNA转录的稳定性.
- 检查了Pat1对具有RNA聚合酶II的基因的招募.
- 研究了转录因子Rlm1和基因素H3位移的招募.
- 分析了Pat1和MAPK Slt2.2的相互依存占用率.
主要成果:
- 缺少Pat1显著影响了细胞壁完整性基因的转录诱导,而不会影响转录稳定性.
- 在细胞壁应激期间,Pat1被招募到细胞壁完整性响应基因中,与RNA Pol II一起.
- Pat1参与了初始化前的复杂组合和转录延长.
- 缺少Pat1的菌株显示Rlm1的招募减少,基因素H3的位移减少,RNA Pol II的进展受损.
- Pat1和Slt2在基因编码区域中表现出相互依赖的占用.
结论:
- 在细胞壁压力下,Pat1作为细胞壁完整性反应基因的转录调节剂.
- 包括Pat1在内的衰变因子可以作为转录调节剂发挥作用.
- 这突显了mRNA衰变机制在应激反应期间的基因调节中的新作用.
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