CeA中的多个代谢信号调节食:AMPKK的作用
Gisele Castro1, Natália Ferreira Mendes2, Laís Weissmann1
1Department of Internal Medicine, School of Medical Science, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
Molecular and cellular endocrinology
|April 11, 2024
概括
这项研究表明,桃体 (CeA) 中央核中的AMP激活蛋白激酶 (AMPK) 在调节对 ghrelin 和禁食的反应中对食物摄入起作用. 在CeA中抑制AMPK可能会降低养行为.
科学领域:
- 神经科学是一个神经科学.
- 代谢调节 代谢调节 代谢调节 代谢调节
- 内分泌学 在内分泌学.
背景情况:
- 杏仁体 (CeA) 的中心核与能量平衡和奖励系统有关.
- 格林素和禁食刺激食的精确信号通路尚不清楚.
- 已知AMP激活蛋白激酶 (AMPK) 是一个细胞能量传感器,可以调节下丘脑中的食物摄入量,但其在CeA中的作用尚未被探索.
研究的目的:
- 为了研究AMPK在CeA中的激活,以对 ghrelin,禁食和2-deoxy-D-glucose (2DG) 的反应.
- 确定养行为是否与CeA中AMPK激活的变化相关.
- 评估CEA中抑制AMPK对食物摄入的影响.
主要方法:
- 在Wistar大鼠中,对CeA的立体感定律.
- 调节剂的使用针对AMPKα1/2Thr172酸化.
- 生理和分子测定测量养,血糖和AMPK激活.
主要成果:
- 禁食在CeA中提高了AMPKThr172,而再养则降低了它.
- 在CeA内给予格林,增加了食物摄入量和AMPKThr172.
- 在CeA中抑制AMPK与Melanotan II (MTII) 减少了体质和七天的食物摄入量.
结论:
- 在CeA中的AMPK激活与控制食的神经回路有关.
- 这些发现有助于理解改变的养行为,如暴饮暴食.
- CeA的AMPK通路对激素和营养素作出反应,影响养控制.
相关概念视频
Regulation of Food Intake
227
Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...
227
cAMP-dependent Protein Kinase Pathways
6.3K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
6.3K
Interactions Between Signaling Pathways
6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K
Amplifying Signals via Enzymatic Cascade
8.5K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
8.5K
Intracellular Signaling Cascades
46.8K
Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
46.8K
Calmodulin-dependent Signaling
5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K


