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来自tambaqui,Colossoma macropomum的冷藏血液和冷血样本的最大储存时间

Marcela C Sena¹, Fábio C Sterzelecki², Paola Fabiana F Gomes²

¹Laboratory of in Vitro Fertilization, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil.

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology

|April 11, 2024

概括

对于tambaqui鱼,冷藏的全血应在24小时内进行分析. 最好在8周内分析冷血样本,以确保准确的健康监测结果.

科学领域:

水生动物的健康状况.
鱼类生理学鱼类生理学
兽医诊断兽医诊断兽医诊断兽医诊断

背景情况:

准确的鱼类健康评估依赖于及时进行血液分析.
样品储存时间可以显著影响血液学和生物化学参数.

研究的目的:

为了确定冷藏的tambaqui (Colossoma macropomum) 全血和冷血的最佳储存时间.
为了在长时间的储存期间识别血液关键参数的潜在变化.

主要方法:

冷藏的全血样本 (4°C) 在0,24,48,72,96小时后进行分析.
冷血样本 (-20°C) 在0,8,12,16和20周后进行分析.
测量了主要的血液学和生物化学参数,包括血红素,血红素,红细胞和白细胞计数,葡萄糖,总蛋白质,甘油三,胆固醇和酶活性 (ALT,AST).

主要成果:

冷藏全血显示MCHC的短暂变化和WBC,TC,葡萄糖和TG的显著下降72-96小时.
红细胞数量在48小时后减少,而ALT在96小时后显著增加.
冷血在8周后显示出显著的ALT变化,并在20周后增加TP和TG.

结论:

为了获得可靠的结果,冷藏的tambaqui全血样本应在24小时内进行分析.
冷的tambaqui血样本应在8周内进行分析,以保持参数完整性.
这些发现支持鱼类血液样本处理和分析的标准化协议.

关键词:

血液参数血液参数鱼类鱼类鱼类鱼类温度储存器的温度储存器是什么?热带鱼是一种热带鱼.

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