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缺氧增加了人类精子的自水平

Jie Hu¹, Jiwei Wu¹, Xinge Liu¹

¹School of Public Health, Guilin Medical University, Zhiyuan Road, Lingui District, Guilin, 541199, Guangxi, China.

Scientific reports

|April 11, 2024

概括

缺氧会增加精子自,并通过HIF-1信号和糖解来降低精子的活力. 希斯H4 ,甲素L和ENO1可能作为阿斯诺精子自和精子活力的生物标志物.

科学领域:

生殖生物学生殖生物学
细胞生理学细胞生理学
生物化学生物化学

背景情况:

氧气感应对于精子功能至关重要.
自对精子质量起着重要作用.
精性精症的特征是精子运动率降低.

研究的目的:

研究人类精子中氧气感应和自的关系.
在低氧条件下识别类精子中差异表达的蛋白质.
探索精子自和活力的潜在生物标志物.

主要方法:

用低氧诱导因子-1α (HIF-1α) 调制剂 (lificiguat/YC-1 或化/CoCl2) 化健康和精精的精液.
无标签的定量蛋白质组分析,以确定差异表达的蛋白质.
与酶相关的免疫吸收试验 (ELISA) 用于选定蛋白质的验证.

主要成果:

缺氧增加了精子自水平,降低了精子活力.
蛋白质组分析揭示了与内 плазма网膜处理,Th17细胞分化,卵细胞成熟和关键代谢途径 (糖解/葡萄糖生成,HIF-1信号) 相关的蛋白质的显著变化.
HIF-1α,LC3B,基因素H4,甲素L和ENO1的表达水平发生了显著变化.

结论:

缺氧增强精子自,并通过HIF-1和糖解/葡萄糖生成信号通路减少活力.
希斯H4,甲素L,谷氨酸合成酶和ENO1被提议作为潜在的生物标志物,用于阿斯类精子的精子自和活力.

关键词:

动物精子缺血症 (Asthenozoospermia) 是一个疾病.自自是一种自的过程.低氧诱导因子-1α.蛋白质组学是指蛋白质组学.精子动物 (Spermatozoa) 是一种精子.

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