一个DNA转位子的多个水平转移事件发生在海,鱼类和青中
Nozhat T Hassan1, James D Galbraith2, David L Adelson3
1School of Biological Sciences, University of Adelaide, Adelaide, Australia.
Mobile DNA
|April 11, 2024
概括
在海中观察到可转移元素的水平转移 (HTT),涉及与鱼和青共享的DNA转移子. 这表明,尽管它们的进化速度缓慢,但的基因组中最近发生了功能性HTT事件.
科学领域:
- 基因组学就是基因组学.
- 进化生物学 进化生物学
- 分子生物学分子生物学
背景情况:
- 可转移元素的水平转移 (HTT) 是一种已知的影响基因组进化的现象.
- 爬行动物基因组,特别是乌 (Testudines) 的基因组,对于HTT事件仍未得到充分研究.
- 可转移元素 (TE) 可以显著改变基因组结构和功能.
研究的目的:
- 为了研究钻石背乌 (Malaclemys terrapin terrapin) 的重复性DNA含量.
- 在海基因组中识别和描述DNA转子的水平转移事件.
- 评估已识别的可转移元素的潜在功能活动.
主要方法:
- 在Malaclemys terrapin terrapin基因组中进行重复元素分析.
- 跨物种DNA转位子序列的生物信息比较.
- 对转移酶蛋白的结构分析,以推断其功能活性.
主要成果:
- 一种非常相似的DNA转子子,hAT-6_XT,在多种乌物种,光鱼和青中被发现.
- 像鱼和鸟类这样的近亲爬行动物中没有hAT-6_XT.
- 结构分析表明转化酶中存在保留的残留物,这表明转化能力保留.
- 这种DNA转位子最近的六个独立的HTT事件在海中被记录下来.
结论:
- 这项研究提供了证据,证明最近和正在进行的DNA转位子水平转移到海基因组中.
- 这些发现凸显了TE进化的动态性质,即使在基因组进化速度缓慢的物种中也是如此.
- hAT-6_XT代表了跨系水平基因转移的重要例子,具有潜在的功能影响.
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