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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Jun 28, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

对于RNA in situ测序的空间分析工具包.

Jiayu Chen¹, Rongqin Ke¹

¹School of Medicine, Huaqiao University, Xiamen, Fujian, China.

Wiley interdisciplinary reviews. RNA

|April 12, 2024

概括

本综述涵盖了用于现场测序 (ISS) 数据分析的生物信息工具,空间转录学 (ST) 方法. 它详细介绍了分析国际空间站数据的工作流程,以了解组织环境中的基因表达.

科学领域:

分子生物学分子生物学
生物信息学是一种生物信息学.
基因组学就是基因组学.

背景情况:

空间转录学 (ST) 能够在本地组织环境中进行基因表达分析,这对于研究基因调节网络至关重要.
现场测序 (ISS) 是一种高分辨率的ST技术,可以在亚细胞水平上检测数百到数千个基因.
对国际空间站日益增长的兴趣需要可扩展的,集成的数据分析工作流程,用于各种研究应用.

研究的目的:

展示最先进的生物信息工具包,用于分析现场测序 (ISS) 数据.
为研究界审查各种工具与国际空间站数据的应用和兼容性.
讨论将ISS分析工具整合到用户友好的管道中的未来前景和挑战.

主要方法:

对现有用于ISS数据分析的生物信息工具包进行全面审查.
详细检查上游和下游分析工作流程,包括图像分析,细胞细分,聚类,功能丰富和空间分析.
评估工具兼容性和专门用于ISS数据的应用.

主要成果:

识别和分类用于ISS数据分析的当前生物信息工具.
详细审查工具功能,涵盖图像处理到先进的空间分析,如细胞相互作用和轨迹推断.
对ISS数据的工具适用性和整合挑战的评估.

关键词:

数据分析数据分析数据分析基因表达分析基因表达分析在现场测序测序.空间转录学空间转录学

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

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Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

相关实验视频

Last Updated: Jun 28, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

结论:

有效的生物信息工具对于释放基于国际空间站的空间转录组学的全部潜力至关重要.
需要标准化和综合分析管道,以促进国际空间站技术在各个研究领域的更广泛采用.
应对当前的挑战将为更容易获得和更强大的ISS数据分析铺平道路.