相关实验视频

Updated: Apr 8, 2026

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Published on: May 4, 2018

在Paenibacillus polymyxa DSM 365中进行基因组缩小,用于底盘开发

Giulia Ravagnan¹, Janne Lesemann¹, Moritz-Fabian Müller²

¹Institute of Molecular Microbiology and Biotechnology, University of Münster, Münster, Germany.

Frontiers in bioengineering and biotechnology

|April 12, 2024

概括

我们向您介绍了Paenibacillus polymyxa DSM 365的第一个完整的基因组序列,这是一种用于生物技术的强壮微生物. 开发了基因组减少策略,为增强生物生产创造了可行的底盘变体.

科学领域:

微生物生物技术和合成生物学
基因组学和基因组工程

背景情况:

Paenibacillus polymyxa DSM 365是一种代谢多功能微生物,具有生物技术应用的潜力.
开发强大的微生物平台对于取代基于化石的工艺至关重要.
完整的基因组序列对于将Paenibacillus polymyxa工程化为微生物底盘至关重要.

研究的目的:

报告Paenibacillus polymyxa DSM 365的第一个完整的基因组序列.
为了改进生物生产,设计Paenibacillus polymyxa的基因组缩小型变种.
为了验证工程菌株适合进一步的基因改造.

主要方法:

全基因组测序和Paenibacillus polymyxa DSM 365的组装.
单一淘汰突变和基因组缩小变体 (GR1,GR2) 的构建.
评估工程菌株的生长特征和产量标题.

主要成果:

为Paenibacillus polymyxa DSM 365建立了一个封闭的基因组序列 (5,889,536 bp).
两种基因组缩小型变异 (GR1和GR2) 已成功创建,基因组大小减少 (3.0%和0.6%).
工程变种表现出野生类型的生长和不受影响的2,3-butanediol和外聚糖的生产.

结论:

完整的基因组序列为Paenibacillus polymyxa DSM 365作为微生物底盘提供了基础.
基因组缩小策略可以产生适合工业生物技术的可行底盘变体.
工程菌株被验证用于进一步的基因工程和生物生产优化.

关键词:

2,3-布坦二醇的使用方法 BGCS BGCS 的意思是帕尼巴西勒斯 (Paenibacillus polymyxa) 是一个多种类型的菌株.底盘底盘底盘底盘基因组的缩小是基因组的缩小.

更多相关视频

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Published on: January 8, 2020

Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility

Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility

Published on: March 11, 2022

相关概念视频

Production of Antibiotics

Production of Antibiotics

Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...