时间依赖的神经元迁移受Cdk5介导的JIP1酸化调节
Qinglin Fei1,2,3,4, Doo Soon Im5, Yiwen Xu6
1Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China.
Frontiers in cell and developmental biology
|April 12, 2024
概括
循环素依赖性激酶5 (Cdk5),p35和JIP1在发育中的脑细胞中被动态表达. 化JIP1在大脑皮层发育过程中影响神经元迁移.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 哺乳动物大脑皮层在进化过程中增加了大小和复杂性.
- 适当的皮质发育依赖于协调的细胞分化和迁移,以精确地形成六层结构.
- 之前的工作将Cdk5介导的JIP1 (JNK相互作用蛋白1) 在T205的酸化与轴突外生长联系起来,但其在皮层发育中的作用尚不清楚.
研究的目的:
- 在小鼠皮层发育过程中,研究Cdk5,p35 (Cdk5r1) 和JIP1 (Mapk8ip1) 在不同细胞类型中的时空表达模式和功能.
- 阐明Cdk5介导的JIP1酸化在神经元迁移和分化中的作用.
主要方法:
- 来自小鼠胚胎皮质的单细胞RNA测序数据的分析.
- 伪素体分析,以追踪细胞发育期间的基因表达动态.
- 在子宫内进行电穿孔来操纵JIP1和相仿JIP1在发育中的神经元中的表达.
主要成果:
- 发现Cdk5,p35和JIP1在中间原始体 (IP) 中被动态表达.
- 这三种基因的表达在神经元迁移和分化过程中,在IP中同时受到上调.
- 化JIP1在T205被证明会影响神经元的时间迁移.
结论:
- Cdk5,p35和JIP1在大脑皮层的发展中起着至关重要的作用.
- 这些基因在中间祖先中的动态表达与神经元迁移和分化有关.
- 由Cdk5在T205处对JIP1的酸化是影响神经元迁移时间的关键调节机制.
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