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双相似物降低了精子干细胞自我更新潜力的调节

Seo-Hee Kim¹, Seung Hee Shin¹, Seok-Man Kim¹

¹Department of Animal Science and Technology, Chung-Ang University, Anseong, Korea.

The world journal of men's health

|April 12, 2024

概括

双A (BPA) 和其替代品双F (BPF) 和双S (BPS) 伤害精子干细胞 (SSC). 这些化学物质减少了SSC种群,可能会影响男性生育能力.

科学领域:

生殖毒理学生殖毒理学
干细胞生物学干细胞生物学

背景情况:

双甲 (BPA) 是一种内分泌干扰化学物质,已知对男性生殖健康有不良影响.
越来越多地使用BPA替代品,如双-F (BPF) 和双-S (BPS),引发了人们对其潜在毒性的担忧.

研究的目的:

研究BPA,BPF和BPS对精子干细胞 (SSC) 种群的影响.
为了比较BPA及其类似物在SSC上的细胞毒性和功能影响,使用体外和体内模型.

主要方法:

从小鼠的丸中分离出SSC,并用不同度的BPA,BPF和BPS进行处理.
使用流式细胞计,免疫细胞化学,RT-qPCR和西部斑点来评估SSC特征和细胞毒性.
进行了体内移植试验,以评估双暴露对SSC和精子生成的功能影响.

主要成果:

BPF在SSC表现出类似于BPA的抑制作用,降低生存率,增殖率和诱导亡.
BPS也表现出显著的SSC细胞毒性,尽管在较小程度上比BPA和BPF.
暴露于BPA,BPF和BPS导致功能性SSCs的减少,这表明移植后生殖细胞殖民地形成的减少.

结论:

BPA的替代品BPF和BPS对SSC具有显著的细胞毒性作用,与BPA相似.
这些发现表明,BPF和BPS可以减少功能性SSC池,可能会影响男性生育能力.

关键词:

双 A 是一种双.双二醇 F 是一种双二醇双 S 是一种双醇.男人生育能力男人生育能力自我更新是一种自我更新.精子干细胞 (Spermatogonial stem cells) 是一种精子干细胞.

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