转录因子PgNAC72激活了DAMMARENEDIOL SYNTHASE的表达,从而促进了人参的氨酸生物合成
Ting Jiang1, Yue Zhang1, Gege Zuo1
1Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha 410008, China.
Plant physiology
|April 12, 2024
概括
甲基斯酸盐 (MeJA) 增强了人参中的女性化物产量. 一个新发现的NAC转录因子,PgNAC72,激活关键酶,增强人参酸生物合成,并为代谢工程提供一个目标.
科学领域:
- 植物分子生物学 植物分子生物学
- 生物化学 生物化学
- 代谢工程是代谢工程.
背景情况:
- 金色化物是人参中重要的生物活性化合物,具有重要的市场价值.
- 甲基斯酸盐 (MeJA) 治疗刺激了人参化物生物合成,但调节途径尚未完全理解.
- NAC转录因子在植物发育和应激反应中起着至关重要的作用.
研究的目的:
- 为了识别和表征MeJA-响应的NAC转录因子,参与Panax人参中的人参酸生物合成.
- 阐明PgNAC72响应MeJA的调节机制及其对人参化物生产的影响.
主要方法:
- 对MeJA处理的P.人参的转录组分析,以确定候选基因.
- 基因克隆,表达分析和PgNAC72.7的亚细胞定位.
- 在P. ginseng callus中过度表达PgNAC72,并随后分析了人参含量.
- 电泳运动转移试验 (EMSA) 和双酶记者试验以确认促进体结合和转录激活.
- 过度表达线的RNA测序和 terpenoid代谢概况.
主要成果:
- 在P. ginseng中发现了一种新的MeJA-响应NAC转录因子基因,PgNAC72.
- PgNAC72局限于细胞核,主要表达在花朵中.
- 过度表达PgNAC72显著增加了总沙波宁和达马兰类型的金氏化物水平.
- PgNAC72直接激活dammaranediol合成酶 (PgDDS) 的促进体,这是人参化物生物合成中的关键酶.
- RNA-seq和代谢学数据证实了OE-PgNAC72系中增强的金氏化物生物合成.
结论:
- 在P.人参中,PgNAC72在MeJA诱导的人参酸生物合成中发挥着关键作用.
- 通过直接调节PgDDS基因,PgNAC72激活了人参化物生产.
- 这项研究提供了对金氏酸调节网络的宝贵见解,并确定PgNAC72是改进金氏酸产量的代谢工程潜在目标.
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