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一个快速而简单的程序,用于对小鱼的整体骨染色

Narawadee Yeesan¹, Chanyut Sudtongkong¹, Supparat Kong-Oh¹

¹Department of Marine Science and Environment, Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang, Thailand.

Journal of histotechnology

|April 12, 2024

概括

一种新的,快速的全挂骨染色技术简化了鱼类研究. 这种更快的骨分析方法有助于生物学海洋学的进化和生态研究.

科学领域:

鱼类学鱼类学鱼类学
生物海洋学生物海洋学
脊椎动物解剖学脊椎动物解剖学

背景情况:

鱼的骨结构为进化和生态学提供了关键的见解.
传统的全身骨染色方法耗时,通常需要数周到数月的时间.
改善染色方案对于推进鱼类研究至关重要.

研究的目的:

为小型鱼类开发一个快速和简化的全挂骨染色协议.
为长时间的传统染色方法提供替代品.

主要方法:

一种新的固定剂被设计并应用于小鱼标本.
新的协议被用于整体骨染色.

主要成果:

新的协议显著减少了骨染色所需的时间.
这种方法很简单,易于采用.
对小河口鱼和其他小脊椎动物的成功应用.

结论:

这种新技术为鱼类骨分析提供了快速有效的方法.
简化协议可以使鱼类学和生态学研究受益.
该方法适用于各种小型脊椎动物物种.

关键词:

骨架系统骨架系统泰国泰国泰国泰国小型河口鱼是一种小鱼.整体装载的骨染色

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Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...