Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Human pluripotent stem cell engineering with CRISPR-Cas9 for Parkinson's disease.

Experimental & molecular medicine·2026
Same author

Materials strategies and multidisciplinary approaches for low-temperature preservation of 3D cellular systems.

Biomedical engineering letters·2026
Same author

Molecular Probes for Sulfatase Detection and Bio-Imaging.

Chembiochem : a European journal of chemical biology·2026
Same author

Associations of fall experiences with cognitive function and activities of daily living disability among older adults: A cross-sectional study.

Journal of Korean gerontological nursing·2026
Same author

Side Hop Test as a Predictor of Successful Return to Sports After Nonoperative Treatment or Modified Broström Operation in Patients With Chronic Ankle Instability: Based on the Ankle-GO Score.

Orthopaedic journal of sports medicine·2026
Same author

Auditory categories in the nonhuman primate.

Handbook of behavioral neuroscience·2026

相关实验视频

Updated: Jun 28, 2025

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound
12:23

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound

Published on: August 14, 2012

14.3K

人类软组织肉瘤分离激活的泛COX成像剂

Kyung Tae Hong1,2, Seung Bin Park3, Dhiraj P Murale2

  • 1Bio-Med Program, KIST-School UST, Hwarang-ro 14 gil 5, Seongbuk-gu, Seoul, 02792, South Korea.

Angewandte Chemie (International ed. in English)
|April 12, 2024
PubMed
概括

新的光探针准循环氧化酶 (COX),以识别瘤中的癌症干细胞. 这种分解激活的传感器显示出对肉瘤组织临床病理成像的前景.

关键词:
身体健康 身体健康在COX-2中,COX-2是最常见的.癌症干细胞癌症干细胞化学传感器是一种化学传感器.拆分 分散 分散 分散.

更多相关视频

Dynamic Contrast Enhanced Magnetic Resonance Imaging of an Orthotopic Pancreatic Cancer Mouse Model
06:24

Dynamic Contrast Enhanced Magnetic Resonance Imaging of an Orthotopic Pancreatic Cancer Mouse Model

Published on: April 18, 2015

15.1K
In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles
09:04

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

Published on: May 2, 2014

11.4K

相关实验视频

Last Updated: Jun 28, 2025

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound
12:23

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound

Published on: August 14, 2012

14.3K
Dynamic Contrast Enhanced Magnetic Resonance Imaging of an Orthotopic Pancreatic Cancer Mouse Model
06:24

Dynamic Contrast Enhanced Magnetic Resonance Imaging of an Orthotopic Pancreatic Cancer Mouse Model

Published on: April 18, 2015

15.1K
In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles
09:04

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

Published on: May 2, 2014

11.4K

科学领域:

  • 生物医学工程 生物医学工程
  • 化学生物学 化学生物学
  • 在瘤学瘤学.

背景情况:

  • 癌症干细胞 (CSCs) 驱动瘤的启动,生长和转移.
  • 在软组织肉瘤等异质恶性瘤中识别CSC是具有挑战性的,因为常规标记物过度表达.
  • 环氧化原酶 (COX) 是肉瘤中潜在的CSC标记物,但需要特定的检测方法.

研究的目的:

  • 设计和合成基于BODIPY的新型光探针 (BD-IMC-1,BD-IMC-2),用于检测循环氧化酶 (COX).
  • 评估探针准和成像肉瘤中CSCs的能力,特别是COX-2阳性细胞.
  • 评估探针在固定的人类肉瘤组织中临床病理应用的潜力.

主要方法:

  • 设计用于分解激活的BODIPY支架光探针 (BD-IMC-1,BD-IMC-2) 的合成.
  • 对探头分解易感性和与COX酶的选择性相互作用的评估.
  • 在活细胞系 (RAW264.7,HeLa,Saos-LM2) 和固定人肉瘤组织中对COX-2上调调节的探针光激活反应的评估.
  • 对探针的毒性评估.

主要成果:

  • 与BD-IMC-2相比,BD-IMC-1表现出更高的分解易感性和与COX的选择性相互作用.
  • 在微小分子以下的度下,BD-IMC-1 证明了对COX-2 的积极合作结合.
  • 这两种探测器都显示出高调节COX-2的活细胞中显著的光"激活" (多达2倍),毒性可忽略不计.
  • BD-IMC-1成功地在巴拉固定的人类肉瘤组织中成像了COX-2阳性细胞.

结论:

  • 分解激活光探针,特别是BD-IMC-1,可以有效地准并对活细胞和固定肉瘤组织中的COX-2进行成像.
  • 这些探针提供了一种敏感和选择性的方法,用于在临床病理学中识别潜在的癌症干细胞.
  • 开发的化学传感器具有在肉瘤研究和临床实践中推进诊断应用的巨大潜力.